概述

  • 产品名称Anti-SAA4抗体
    参阅全部 SAA4 一抗
  • 描述
    小鼠多克隆抗体to SAA4
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-130 of Human SAA4

  • 阳性对照
    • Human stomach tissue lysate; SAA4 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab67358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

靶标

  • 功能Major acute phase reactant. Apolipoprotein of the HDL complex.
  • 组织特异性Expressed by the liver; secreted in plasma.
  • 序列相似性Belongs to the SAA family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amyloid A, serum, 4 antibody
    • C SAA antibody
    • C-SAA antibody
    • Constitutively expressed serum amyloid A protein antibody
    • CSAA antibody
    • SAA 4 antibody
    • Saa4 antibody
    • SAA4 protein antibody
    • SAA4_HUMAN antibody
    • Serum amyloid A 4 protein antibody
    • Serum amyloid A-4 protein antibody
    • Serum amyloid A4 constitutive antibody
    see all

Anti-SAA4 antibody 图像

  • Anti-SAA4 antibody (ab67358) at 1/500 dilution + Human stomach tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 15 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 13 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SAA4 antibody (ab67358) at 1/500 dilution

    Lane 1 : SAA4 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 15 kDa
    Observed band size : 13 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab67358 staining in human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67358, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SAA4 antibody (ab67358)参考文献

ab67358 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (whole human plasma)
Loading amount 14 µg
Specification whole human plasma
Treatment heated to 95C in 10-fold excess of reducing SDS sample buffer for 10 min
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Mar 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"