概述

  • 产品名称Anti-SA2抗体
    参阅全部 SA2 一抗
  • 描述
    山羊多克隆抗体to SA2
  • 特异性We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human SA2.

性能

应用

Our Abpromise guarantee covers the use of ab4463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
ICC 1/500 - 1/2000.
ICC/IF 1/200.

靶标

  • 功能Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • 序列相似性Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • 翻译后修饰Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • 细胞定位Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA517O1.1 antibody
    • Cohesin Subunit SA 2 antibody
    • Cohesin subunit SA-2 antibody
    • DKFZp686P168 antibody
    • DKFZp781H1753 antibody
    • FLJ25871 antibody
    • SA 2 antibody
    • SA-2 antibody
    • SA2 antibody
    • SCC3 homolog 2 antibody
    • SCC3B antibody
    • STAG 2 antibody
    • stag2 antibody
    • STAG2_HUMAN antibody
    • Stromal antigen 2 antibody
    see all

Anti-SA2 antibody 图像

  • Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4463 at 1µg/ml.


  • Predicted band size : 134 kDa

    Nuclear Extract from HeLa cells (~5 x 105 cells).

    ab4463 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.

    Nuclear Extract from HeLa cells (~5 x 10e5 cells).

    ab4463 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.
  • Goat polyclonal to SA2 antibody (ab4463) detecting Stromal antigen 2 in assynchronous HeLa cells, together with a Donkey anti-goat secondary antibody conjugated to Cy3 and DAPI as counterstain. Please refer to abreview for further protocol details.

    See Abreview

Anti-SA2 antibody (ab4463)参考文献

This product has been referenced in:
  • Ishiguro K  et al. A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing. EMBO Rep 12:267-75 (2011). WB ; Mouse . Read more (PubMed: 21274006) »
  • Xiao T  et al. Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity. Mol Cell Biol 31:2174-83 (2011). WB ; Human . Read more (PubMed: 21444719) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Apr 16 2007

Thank you for your email. All the information that we have regarding these antibodies is located on the online datasheets. I unfortunately can't tell you which one works "best." Are you detecting SA1 or SA2? The differences between the two SA1 antibodi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"