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The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 424 (P-V-SP-P-V).
Our Abpromise guarantee covers the use of ab47379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).|
|IHC-P||Use at an assay dependent concentration.|
ICC/IF image of ab47379 stained DU145 cells. The cells were 10% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab47379 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.