RabMAb

Anti-S100P抗体[EPR6142] (ab124743)

概述

  • 产品名称Anti-S100P抗体[EPR6142]
    参阅全部 S100P 一抗
  • 描述
    兔单克隆抗体[EPR6142] to S100P
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human S100P aa 1-100.

  • 阳性对照
    • BxPC3 cell lysate; Human pancreatic adenocarcinoma, placenta and spleen tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-S100P antibody (Alexa Fluor® 488) [EPR6142] (ab207659)

    Anti-S100P antibody (Alexa Fluor® 647) [EPR6142] (ab207660)

性能

应用

Our Abpromise guarantee covers the use of ab124743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P 1/150 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/150.

靶标

  • 组织特异性Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias.
  • 序列相似性Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 细胞定位Nucleus. Cytoplasm. Colocalizes with S100PBP in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MIG9 antibody
    • Migration inducing gene 9 antibody
    • Protein S100-E antibody
    • Protein S100-P antibody
    • Protein S100P antibody
    • S100 calcium binding protein P antibody
    • S100 calcium-binding protein P antibody
    • S100 P antibody
    • S100E antibody
    • S100P antibody
    • S100P_HUMAN antibody
    see all

Anti-S100P antibody [EPR6142] 图像

  • Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution (purified) + BxPC-3 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 10 kDa
    Observed band size : 10 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human pancreatic adenocarcinoma with purified ab124743 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunofluorescence staining of BxPC-3 cells with purified ab124743 at a working dilution of 1 in 150, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti-rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab124743 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500.

  • Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution (unpurified) + BxPC3 cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 10 kDa
    Observed band size : 10 kDa
  • Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human pancreatic adenocarcinoma tissue by immunohistochemistry.

  • Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human placenta tissue by immunohistochemistry.

  • Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human spleen tissue by immunohistochemistry.

  • Unpurified ab124743 showing negative staining in normal liver tissue.

  • Unpurified ab124743 showing positive staining in colonic adenocarcinoma tissue.

  • Unpurified ab124743 showing negative staining in normal pancreas tissue.

Anti-S100P antibody [EPR6142] (ab124743)参考文献

ab124743 has not yet been referenced specifically in any publications.

Product Wall

Thanks for contacting us. The immunogen for ab124743 Anti-S100P, has 56% sequence similarity with S100A1, and 33% with S100B. Therefore, it is unlikely that the antibody will cross react with either of these proteins.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"