概述

  • 产品名称Anti-S100A9抗体
    参阅全部 S100A9 一抗
  • 描述
    山羊多克隆抗体to S100A9
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Cow
  • 免疫原

    Synthetic peptide:

    C-DTNADKQLSFEEF

    , corresponding to internal sequence amino acids 67-79 of Human S100A9 according to NP_002956.1

  • 阳性对照
    • Human Peripheral Mononucleocytes lysate

性能

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应用

Our Abpromise guarantee covers the use of ab81522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明Peptide ELISA: antibody detection limit dilution: 1/8000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 13 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
    • 组织特异性Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
    • 序列相似性Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • 翻译后修饰Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
    • 细胞定位Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Leukocyte L1 complex heavy chain antibody
      • 60B8AG antibody
      • CAGB antibody
      • Calgranulin B antibody
      • Calgranulin-B antibody
      • Calprotectin L1H subunit antibody
      • CFAG antibody
      • CGLB antibody
      • Cystic fibrosis antigen B antibody
      • L1AG antibody
      • Leukocyte L1 complex heavy chain antibody
      • LIAG antibody
      • MAC387 antibody
      • MIF antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor-related protein 14 antibody
      • MRP 14 antibody
      • MRP-14 antibody
      • MRP14 antibody
      • Myeloid-related protein 14 antibody
      • NIF antibody
      • OTTHUMP00000015331 antibody
      • p14 antibody
      • Protein S100-A9 antibody
      • S100 A9 antibody
      • S100 calcium binding protein A9 antibody
      • S100 calcium binding protein A9 calgranulin B antibody
      • S100 calcium-binding protein A9 antibody
      • S100A9 antibody
      • S10A9_HUMAN antibody
      see all

    Anti-S100A9 antibody 图像

    • Anti-S100A9 antibody (ab81522) at 0.5 µg/ml (for 1 hour) + Human Peripheral Mononucleocytes lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 13 kDa
      Observed band size : 16 kDa (why is the actual band size different from the predicted?)

    Anti-S100A9 antibody (ab81522)参考文献

    This product has been referenced in:
    • Tsai SY  et al. DAMP molecule S100A9 acts as a molecular pattern to enhance inflammation during influenza A virus infection: role of DDX21-TRIF-TLR4-MyD88 pathway. PLoS Pathog 10:e1003848 (2014). ELISA . Read more (PubMed: 24391503) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"