Anti-S100A9抗体[47-8D3] (ab24111)

概述

  • 产品名称
    Anti-S100A9抗体[47-8D3]
    参阅全部 S100A9 一抗
  • 描述
    小鼠单克隆抗体[47-8D3] to S100A9
  • 经测试应用
    适用于: IHC-Fr, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation of peripheral blood monocyte components (Human).

  • 阳性对照
    • Lysate of human monocytes or granulocytes.
  • 常规说明


    Can be used for phenotypical characterization of macrophages and myelomonocytic cells in situ.

性能

应用

Our Abpromise guarantee covers the use of ab24111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 14 kDa.

靶标

  • 功能
    Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • 组织特异性
    Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
  • 序列相似性
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 翻译后修饰
    Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
  • 细胞定位
    Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Leukocyte L1 complex heavy chain antibody
    • 60B8AG antibody
    • CAGB antibody
    • Calgranulin B antibody
    • Calgranulin-B antibody
    • Calprotectin L1H subunit antibody
    • CFAG antibody
    • CGLB antibody
    • Cystic fibrosis antigen B antibody
    • L1AG antibody
    • Leukocyte L1 complex heavy chain antibody
    • LIAG antibody
    • MAC387 antibody
    • MIF antibody
    • Migration inhibitory factor related protein 14 antibody
    • Migration inhibitory factor-related protein 14 antibody
    • MRP 14 antibody
    • MRP-14 antibody
    • MRP14 antibody
    • Myeloid-related protein 14 antibody
    • NIF antibody
    • OTTHUMP00000015331 antibody
    • p14 antibody
    • Protein S100-A9 antibody
    • S100 A9 antibody
    • S100 calcium binding protein A9 antibody
    • S100 calcium binding protein A9 calgranulin B antibody
    • S100 calcium-binding protein A9 antibody
    • S100A9 antibody
    • S10A9_HUMAN antibody
    see all

文献

This product has been referenced in:
  • Sill M  et al. Protein Profiling Gastric Cancer and Neighboring Control Tissues Using High-Content Antibody Microarrays. Microarrays (Basel) 5:N/A (2016). IHC . Read more (PubMed: 27600085) »
  • Sun W  et al. Quantitative Proteomics Analysis of Tissue Interstitial Fluid for Identification of Novel Serum Candidate Diagnostic Marker for Hepatocellular Carcinoma. Sci Rep 6:26499 (2016). WB . Read more (PubMed: 27216119) »

See all 6 Publications for this product

客户评价及客户问答

Thank you for contacting us.

We do not have this type of clinical data as our products are for in vitro use. A literature search should be able to provide you with this kind of information.


I hope this information is helpful ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Oral dyplastic epithelium cells)
Specification
Oral dyplastic epithelium cells
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2%
Username

Mr. Cancer center

Verified customer

提交于 Jul 25 2006

Thank you for your enquiry. The antibody reacts with MRP 14 (L1 monocyte antigen), the exact epitope is not known. I hope this information helps. Please do not hesitate to contact us again if you need anyting further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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