Anti-S100A9 + Calprotectin (S100A8/A9 complex)抗体[MAC387] (ab22506)

概述

  • 产品名称Anti-S100A9 + Calprotectin (S100A8/A9 complex)抗体[MAC387]
    参阅全部 S100A9 + Calprotectin (S100A8/A9 complex) 一抗
  • 描述
    小鼠单克隆抗体[MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)
  • 特异性Recognizes S100A9 (also known as MRP-14 or Calgranulin B) and Calprotectin (also known as S100A8/A9 complex or MRP-8/MRP-14 complex). The MAC387 clone has been reported to recognize S100A12 (also known as Calgranulin C). These S100 calcium-binding proteins are expressed by granulocytes, monocytes and macrophages. This clone is also known as Macrophage + Monocytes antibody, anti-Macrophages antibody and Calprotectin antibody.
  • 经测试应用适用于: IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rabbit, Cow, Dog, Human, Pig, Monkey
    预测可用于: Horse, Guinea pig, Cat, Baboon不与反应: Rat
  • 免疫原

    Tissue, cells or virus corresponding to Human S100A9 + Calprotectin.

  • 阳性对照
    • Human spleen

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium azide
    Constituent: PBS

    Some batches may contain 1% BSA. Please contact Scientific Support for batch information.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆单克隆
  • 克隆编号MAC387
  • 骨髓瘤NS1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100 - 1/200.
IHC-P Use a concentration of 1 µg/ml.

Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] 图像

  • ab22506 staining Macrophage in 12um frozen sections of pig skin by Immunohistochemistry (IHC-Fr - frozen sections). Tissue permeabilized with 0..25% Tween20 in TBS and blocked with 5% serum for 60 minutes at 20°C. Samples were incubated with primary antibody (1/200 in TBS 0.02% Triton X-100, 1% BSA) for 16 hours at 4°C. An Alexa®488-conjugated Donkey anti-mouse IgG monoclonal (1/500) was used as the secondary antibody.

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  • ab22506 staining Macrophages in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.250% casein for 5 minutes at 25°C; antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/5000 in Tris-HCL) for 30 minutes at 25°C. A HRP-conjugated labeled polymer was used as the secondary antibody.

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  • IHC image of Macrophage staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab22506 staining Macrophages in human brain tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone and blocked for 30 minutes at 25°C. The primary antibody was diluted, 1/100 and incubated with sample for 1 hours at 37°C. A Biotin conjugated goat polyclonal to mouse IgG1, diluted 1/250 was used as secondary. The Biotinylated sites were visualized with Invitrogen streptavidin-Alexa546.

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  • IHC image of Macrophage staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)参考文献

This product has been referenced in:
  • Nio-Kobayashi J  et al. Histological analysis of arteriovenous anastomosis-like vessels established in the corpus luteum of cows during luteolysis. J Ovarian Res 9:67 (2016). IHC ; Cow . Read more (PubMed: 27756340) »
  • Karna SL  et al. RNA-Seq Transcriptomic Responses of Full-Thickness Dermal Excision Wounds to Pseudomonas aeruginosa Acute and Biofilm Infection. PLoS One 11:e0165312 (2016). IHC ; Rabbit . Read more (PubMed: 27792773) »

See all 23 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Sample Mouse Tissue sections (spleen)
Specification spleen
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 01 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Sample Pig Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 01 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Pig Tissue sections (Skin)
Specification Skin
Permeabilization Yes - 0.05% Tween 20
Fixative 10% Formalin
Username

Abcam user community

Verified customer

提交于 Oct 28 2014

L1 is as an alternative name for Calprotectin. I was also able to locate the correct Swiss Prot Id for you: P05109 (S10A8_HUMAN) http://www.uniprot.org/uniprot/P05109

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Spleen and Aortic Valve Tissue)
Specification Spleen and Aortic Valve Tissue
Fixative 10% NBF
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Trypsin-CaCl 0.001 mg/mL, 30 min at 37*C
Permeabilization No
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Krista Sider

Verified customer

提交于 Jan 21 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (normal and wounded skin)
Specification normal and wounded skin
Fixative no fixation
Permeabilization Yes - 0.25% tween 20 in TBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 15 2012

Thank you for your enquiry and your interest in our products.

I can confirm that ab22506 has only been tested in Mouse, Rabbit, Cow, Dog, Human, Pig, Monkey and due to sequence homology it may work in Horse, Guinea pig, Cat, Baboon. There are...

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Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab56297 on the order ***, which should arrive tomorrow.

Please keep me updated about t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"