Anti-S100A9 + Calprotectin (S100A8/A9 complex)抗体[MAC387] (ab22506)

概述

  • 产品名称
    Anti-S100A9 + Calprotectin (S100A8/A9 complex)抗体[MAC387]
    参阅全部 S100A9 + Calprotectin (S100A8/A9 complex) 一抗
  • 描述
    小鼠单克隆抗体[MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)
  • 特异性
    Recognizes S100A9 (also known as MRP-14 or Calgranulin B) and Calprotectin (also known as S100A8/A9 complex or MRP-8/MRP-14 complex). The MAC387 clone has been reported to recognize S100A12 (also known as Calgranulin C). These S100 calcium-binding proteins are expressed by granulocytes, monocytes and macrophages. This clone is also known as Macrophage + Monocytes antibody, anti-Macrophages antibody and Calprotectin antibody.
  • 经测试应用
    适用于: IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rabbit, Cow, Dog, Human, Pig, Monkey
    预测可用于: Horse, Guinea pig, Cat, Baboon不与反应: Rat
  • 免疫原

    Tissue, cells or virus corresponding to Human S100A9 + Calprotectin.

  • 阳性对照
    • Human spleen
  • 常规说明

    This antibody clone is manufactured by Abcam.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium azide
    Constituent: PBS

    Some batches may contain 1% BSA. Please contact Scientific Support for batch information.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    单克隆
  • 克隆编号
    MAC387
  • 骨髓瘤
    NS1
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100 - 1/200.
IHC-P Use a concentration of 1 µg/ml.

图片

  • ab22506 staining Macrophage in 12um frozen sections of pig skin by Immunohistochemistry (IHC-Fr - frozen sections). Tissue permeabilized with 0..25% Tween20 in TBS and blocked with 5% serum for 60 minutes at 20°C. Samples were incubated with primary antibody (1/200 in TBS 0.02% Triton X-100, 1% BSA) for 16 hours at 4°C. An Alexa®488-conjugated Donkey anti-mouse IgG monoclonal (1/500) was used as the secondary antibody.

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  • ab22506 staining Macrophages in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.250% casein for 5 minutes at 25°C; antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/5000 in Tris-HCL) for 30 minutes at 25°C. A HRP-conjugated labeled polymer was used as the secondary antibody.

    See Abreview

  • IHC image of Macrophage staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab22506 staining Macrophages in human brain tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone and blocked for 30 minutes at 25°C. The primary antibody was diluted, 1/100 and incubated with sample for 1 hours at 37°C. A Biotin conjugated goat polyclonal to mouse IgG1, diluted 1/250 was used as secondary. The Biotinylated sites were visualized with Invitrogen streptavidin-Alexa546.

    See Abreview

  • IHC image of Macrophage staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

文献

This product has been referenced in:
  • Nio-Kobayashi J  et al. Histological analysis of arteriovenous anastomosis-like vessels established in the corpus luteum of cows during luteolysis. J Ovarian Res 9:67 (2016). IHC ; Cow . Read more (PubMed: 27756340) »
  • Karna SL  et al. RNA-Seq Transcriptomic Responses of Full-Thickness Dermal Excision Wounds to Pseudomonas aeruginosa Acute and Biofilm Infection. PLoS One 11:e0165312 (2016). IHC ; Rabbit . Read more (PubMed: 27792773) »

See all 30 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Normal Spleen)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Normal Spleen
Blocking step
Peroxide as blocking agent for 3 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Formaldehyde
Username

Mr. Mauro Frazier

Verified customer

提交于 Aug 15 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 23 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 01 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Sample
Pig Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 01 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Pig Tissue sections (Skin)
Specification
Skin
Permeabilization
Yes - 0.05% Tween 20
Fixative
10% Formalin
Username

Abcam user community

Verified customer

提交于 Oct 28 2014

L1 is as an alternative name for Calprotectin. I was also able to locate the correct Swiss Prot Id for you: P05109 (S10A8_HUMAN) http://www.uniprot.org/uniprot/P05109

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Spleen and Aortic Valve Tissue)
Specification
Spleen and Aortic Valve Tissue
Fixative
10% NBF
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Trypsin-CaCl 0.001 mg/mL, 30 min at 37*C
Permeabilization
No
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Krista Sider

Verified customer

提交于 Jan 21 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (normal and wounded skin)
Specification
normal and wounded skin
Fixative
no fixation
Permeabilization
Yes - 0.25% tween 20 in TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 15 2012

Thank you for your enquiry and your interest in our products.

I can confirm that ab22506 has only been tested in Mouse, Rabbit, Cow, Dog, Human, Pig, Monkey and due to sequence homology it may work in Horse, Guinea pig, Cat, Baboon. There are...

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