The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1. Use neat for 10 min at room temperature. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
Belongs to the S-100 family. Contains 2 EF-hand domains.