概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to S100A4
  • 宿主
    Mouse
  • 经测试应用
    适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein corresponding to amino acids 1-102 (MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT) of Human S100A4 (AAH16300) with a proprietary tag.

  • 阳性对照
    • HeLa cell and NIH/3T3 lysates. Transfected 293T cell line.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab220213 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab93283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 12 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 (FSP1) antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 (MTS1) antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

图片

  • Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa
    Observed band size: 37.22 kDa (why is the actual band size different from the predicted?)



    Predicted band size for the immunogen in 37.22 kDa.
  • Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + HeLa cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa

  • Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + NIH/3T3 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa

  • All lanes : Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer)

    Lane 1 : cell lysate from S100A4 transfected 293T cell line
    Lane 2 : cell lysate from non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa
    Observed band size: 11.7 kDa (why is the actual band size different from the predicted?)

  • ab93283 at 15µg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.
  • ab93283 detecting recombinant S100A4 (with a proprietary tag) by sandwich ELISA. The detection limit for recombinant S100A4 (with a proprietary tag) is approximately 0.1 ng/ml as a capture antibody.

文献

This product has been referenced in:
  • Nguy?n-Thanh T  et al. Inhibition of histone deacetylase 1 ameliorates renal tubulointerstitial fibrosis via modulation of inflammation and extracellular matrix gene transcription in mice. Int J Mol Med 41:95-106 (2018). Read more (PubMed: 29115561) »
  • Wilding C  et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). Read more (PubMed: 24595072) »

See all 2 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated cell)
Specification
differentiated cell
Fixative
Paraformaldehyde
Permeabilization
Yes - TritonX
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Nov 12 2012

Thank you for contacting us and your interest in our products.

I have looked into the three antibodies we have directed against S100A4 and whether they are likely to work when used in a sandwich ELISA. We have the following antibodies directe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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