Anti-S100A4抗体[1F12-1G7] (ab93283)
Key features and details
- Mouse monoclonal [1F12-1G7] to S100A4
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-S100A4抗体[1F12-1G7]
参阅全部 S100A4 一抗 -
描述
小鼠单克隆抗体[1F12-1G7] to S100A4 -
宿主
Mouse -
经测试应用
适用于: ELISA, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to amino acids 1-102 (MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT) of Human S100A4 (AAH16300) with a proprietary tag.
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阳性对照
- HeLa cell and NIH/3T3 lysates. Transfected 293T cell line.
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常规说明
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
1F12-1G7 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93283于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. - Information by UniProt
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数据库链接
- Entrez Gene: 6275 Human
- Entrez Gene: 20198 Mouse
- Omim: 114210 Human
- SwissProt: P26447 Human
- SwissProt: P07091 Mouse
- Unigene: 654444 Human
- Unigene: 3925 Mouse
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别名
- 18A2 antibody
- 42A antibody
- calcium Placental protein antibody
see all
图片
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Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 37.22 kDa why is the actual band size different from the predicted?Predicted band size for the immunogen in 37.22 kDa.
This image was generated using the ascites version of the product.
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Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + HeLa cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDaThis image was generated using the ascites version of the product.
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Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + NIH/3T3 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDaThis image was generated using the ascites version of the product.
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All lanes : Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer)
Lane 1 : cell lysate from S100A4 transfected 293T cell line
Lane 2 : cell lysate from non transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 11.7 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
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ab93283 at 15µg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.
This image was generated using the ascites version of the product.
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Detection limit for recombinant GST tagged S100A4 is 0.1 ng/ml as a capture antibody.
实验方案
数据表及文件
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Datasheet download
文献 (7)
ab93283 被引用在 7 文献中.
- Jia M et al. Inhibition of PI3K/AKT/mTOR Signalling Pathway Activates Autophagy and Suppresses Peritoneal Fibrosis in the Process of Peritoneal Dialysis. Front Physiol 13:778479 (2022). PubMed: 35309056
- Gundlach JP et al. Paracrine Interaction of Cholangiocellular Carcinoma with Cancer-Associated Fibroblasts and Schwann Cells Impact Cell Migration. J Clin Med 11:N/A (2022). PubMed: 35628911
- Ugai K et al. Inhibition of the SET8 Pathway Ameliorates Lung Fibrosis Even Through Fibroblast Dedifferentiation. Front Mol Biosci 7:192 (2020). PubMed: 32850975
- Méndez-Barbero N et al. A major role of TWEAK/Fn14 axis as a therapeutic target for post-angioplasty restenosis. EBioMedicine 46:274-289 (2019). PubMed: 31395500
- Nguy?n-Thanh T et al. Inhibition of histone deacetylase 1 ameliorates renal tubulointerstitial fibrosis via modulation of inflammation and extracellular matrix gene transcription in mice. Int J Mol Med 41:95-106 (2018). PubMed: 29115561
- Zhang W et al. S100a4 Is Secreted by Alternatively Activated Alveolar Macrophages and Promotes Activation of Lung Fibroblasts in Pulmonary Fibrosis. Front Immunol 9:1216 (2018). PubMed: 29910813
- Wilding C et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). PubMed: 24595072