产品名称 Anti-S100A4抗体 参阅全部 S100A4 一抗
特异性 Both variants (NP_002952.1; NP_062427.1) represent identical protein.
IHC-P, WB more details
Mouse, Rat, Dog
CNEFFEGFPDKQP , corresponding to C terminal amino acids 86-98 of Human S100A4
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
组织特异性 Ubiquitously expressed.
序列相似性 Belongs to the S-100 family. Contains 2 EF-hand domains.
Information by UniProt
calcium Placental protein antibody
Anti-S100A4 antibody 图像
Western blot - S100A4 antibody (ab58597)
Anti-S100A4 antibody (ab58597) at 0.03 µg/ml + Peripheral blood mononuclear cell lysate (in RIPA buffer) at 35 µg Predicted band size : 12 kDa Observed band size : 13 kDa ( why is the actual band size different from the predicted?) Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody (ab58597)
ab58597 at a concentration of 2µg/ml staining S100A4 in l
ymphatic cells in the lamina propria of human small intestine tissue sections by immunohistochemistry (paraffin-embedded). Antigen retrieval was with a citrate buffer.
Anti-S100A4 antibody (ab58597)参考文献
This product has been referenced in:
et al. Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target. Mol Cell Proteomics 12:3778-92 (2013).
Read more (PubMed: 24037664) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"