概述

  • 产品名称Anti-S100A4抗体
    参阅全部 S100A4 一抗
  • 描述
    兔多克隆抗体to S100A4
  • 经测试应用适用于: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human S100A4.

    (Peptide available as ab41531.)

  • 阳性对照
    • This antibody gives a positive signal in NIH3T3 whole cell lysate and when tested against Human S100A4 Recombinant Protein. This antibody gives a positive signal in the following formaldehyde fixed cell lines: MCF-7.
  • 常规说明

    Blocking peptide available ab41531.

性能

应用

Our Abpromise guarantee covers the use of ab41532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB 1/250. Predicted molecular weight: 12 kDa.Can be blocked with Human S100A4 peptide (ab41531).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-S100A4 antibody 图像

  • ab41532 staining S100A4 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

  • ICC/IF image of ab41532 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41532 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-S100A4 antibody (ab41532) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
    Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-S100A4 antibody (ab41532) at 1/250 dilution + Recombinant human S100A4 protein (ab83650) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 12 kDa


    Exposure time : 20 minutes

    The S100A4 tagged recombinant protein has a molecular weight of 36.8 kDa.

Anti-S100A4 antibody (ab41532)参考文献

This product has been referenced in:
  • Taniguchi T  et al. Fibrosis, vascular activation, and immune abnormalities resembling systemic sclerosis in bleomycin-treated Fli-1-haploinsufficient mice. Arthritis Rheumatol 67:517-26 (2015). IHC ; Mouse . Read more (PubMed: 25385187) »
  • Crabbé A  et al. Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. PLoS One 10:e0126846 (2015). IHC-P ; Mouse . Read more (PubMed: 25962111) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human umbilical vein endothelial cell)
Specification human umbilical vein endothelial cell
Fixative Cytofix Fixation and Permeabilization
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Mar 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human dermal microvascular endothelial cell)
Loading amount 30 µg
Specification Human dermal microvascular endothelial cell
Treatment 30mM Glucose, 10nG TNF-alpha for 24hrs, 48hrs
Gel Running Conditions Reduced Denaturing (4-20% gradient SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 18 2013

ab83650, S100A4 protein is actually a GST-tagged protein which has a total MW of 36.8 kDa. GST(glutathione S-transferase) which is commonly applied in recombinant protein is about 26kDa. Therefore, S100A4 protein (12kDa) + GST(26kDa) is around 38kDa, a...

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Thank you for your reply.

As agreed I added one unit of ab41532, free of charge, to the order number ***** in compensation of the problem experienced with ab41532 ordered in January 2012.

Please remind your customers that our Abpromis...

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Reply to CCE ******* and CCE ******** as I assume both complaints are from the same customer.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear both antibodies ab41532 and ab106777 are not provi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Cryosection-Heart)
Specification Cryosection-Heart
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 02 2012

According to our records, ab41532 was proving difficult to use and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the d...

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DISCOUNT CODE: ***********
Expiration date: ************

I am very pleased to hear you would like to accept our offer and test ab41532 in sheep tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redee...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with ab41532. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Mr. Antibody Solutions

Verified customer

提交于 Jan 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"