概述

  • 产品名称Anti-S100A4抗体
    参阅全部 S100A4 一抗
  • 描述
    兔多克隆抗体to S100A4
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human S100A4.

    (Peptide available as ab41530.)

  • 阳性对照
    • This antibody gives a positive signal in Human S100A4 Recombinant Protein.
  • 常规说明

    Blocking peptide available ab41530 

性能

应用

Our Abpromise guarantee covers the use of ab40722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 12 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 20551063
IHC-P Use at an assay dependent concentration.

靶标

  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

Anti-S100A4 antibody 图像

  • Anti-S100A4 antibody (ab40722) at 1 µg/ml + S100A4 - Recombinant Protein at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 12 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • ab40722 staining S100A4 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

  • ICC/IF image of ab40722 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-S100A4 antibody (ab40722)参考文献

This product has been referenced in:
  • Nutter F  et al. Different molecular profiles are associated with breast cancer cell homing compared with colonisation of bone: evidence using a novel bone-seeking cell line. Endocr Relat Cancer 21:327-41 (2014). Read more (PubMed: 24413608) »
  • Bockmeyer CL  et al. Arteriolar vascular smooth muscle cell differentiation in benign nephrosclerosis. Nephrol Dial Transplant 27:3493-501 (2012). Read more (PubMed: 22319217) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Sample Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Sep 13 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Both Cit (pH6) and Tris (pH8) similar results
Sample Human Tissue sections (Nerves)
Specification Nerves
Permeabilization No
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Aug 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Mr. Antibody Solutions

Verified customer

提交于 Jan 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (YTS human NK cell line lysate)
Loading amount 1e+006 cells
Specification YTS human NK cell line lysate
Gel Running Conditions Reduced Denaturing (NuPage Bis-Tris 4-12%)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Jan 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"