Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog
A region within synthetic peptide: MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF, corresponding to N terminal amino acids 1-50 of Human S100A3
- Human skin tissue or OVCAR3 cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 5 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 12 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use a concentration of 4 - 8 µg/ml.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
Binds both calcium and zinc. Probably binds 2 zinc ions per molecule. May be involved in calcium-dependent cuticle cell differentiation and hair shaft formation.
Belongs to the S-100 family.
Contains 2 EF-hand domains.
Information by UniProt
- Protein S 100E antibody
- Protein S-100E antibody
- Protein S100 A3 antibody
Western blot - Anti-S100A3 antibody (ab62277)
Anti-S100A3 antibody (ab62277) at 5 µg/ml + OVCAR3 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG diluted in 1: 50,000 - 100,000 Predicted band size:
12 kDaObserved band size:
10 kDa (why is the actual band size different from the predicted?
)Additional bands at:
59 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A3 antibody (ab62277)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling S100A3 with ab62277 at 4-8µg/ml. Arrows indicate positively labelled squamous epithelial cells. Magnification: 400X.
has not yet been referenced specifically in any publications.
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