Anti-S100 beta抗体[EP1576Y] (ab52642)

Blocking and Diluting buffer and concentration: 5% NFDM/TBST

Unpurified ab52642 staining S100 beta in human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor^® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A-375 (human malignant melanoma cell line) cells labeling S100 beta with purified ab52642 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor^® 488) ab150077 secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue). The negative control is as follows;
ab52642 at 1/100 dilution followed by ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.

Immunohistochemical analysis of paraffin embedded human cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

Negative control: Using PBS instead of primary antibody.

Blocking and Diluting buffer and concentration: 5% NFDM/TBST

Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

Negative control: Using PBS instead of primary antibody.

Immunocytochemistry/ Immunofluorescence analysis of mouse colon-derived neurospheres labeling S100 beta with ab52642 at 1/400 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Next the cells were blocked with 5 % serum for 1 hour at 25°C, followed by incubation with anti-S100 beta antibody [EP1576Y] (ab52642) in 1% BSA for 18 hours at 4°C. A polyclonal goat anti-rabbit IgG Alexa Fluor^® 594 was used at 1/200 dilution.

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Blocking  and Diluting buffer and concentration: 5% NFDM/TBST

Immunohistochemical analysis of paraffin embedded mouse cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

Negative control: Using PBS instead of primary antibody.

S100 beta was immunoprecipitated from human fetal brain with purified ab52642 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52642 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Immunohistochemical analysis of embryonic mouse brain tissue, staining S100 beta with unpurified ab52642.