重组
RabMAb

Anti-S100 beta抗体[EP1576Y] (ab52642)

概述

  • 产品名称
    Anti-S100 beta抗体[EP1576Y]
    参阅全部 S100 beta 一抗
  • 描述
    兔单克隆抗体[EP1576Y] to S100 beta
  • 经测试应用
    适用于: ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Goat, Human, Zebrafish, Macaque monkey
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human S100 beta aa 50 to the C-terminus (C terminal).
    Database link: P04271

  • 阳性对照
    • IHC-P: Human, mouse and rat cerebral cortex. Human spiral ganglion and melanoma tissue. WB: B16F0 and A-375 cell lysates, mouse spinal cord, rat brain. ICC/IF: A-375 cell lysates. IP: Human fetal brain.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab52642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/1000 - 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

 

IP 1/50.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

靶标

  • 功能
    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
  • 组织特异性
    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
  • 序列相似性
    Belongs to the S-101 family.
    Contains 2 EF-hand domains.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NEF antibody
    • Protein S100 B antibody
    • Protein S100-B antibody
    • S 100 calcium binding protein beta chain antibody
    • S 100 protein beta chain antibody
    • S-100 protein beta chain antibody
    • S-100 protein subunit beta antibody
    • S100 antibody
    • S100 calcium binding protein beta (neural) antibody
    • S100 calcium-binding protein B antibody
    • S100 protein beta chain antibody
    • S100B antibody
    • S100B_HUMAN antibody
    • S100beta antibody
    see all

图片

  • Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/5000 dilution (purified) + A-375 (human malignant melanoma cell line) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

  • Unpurified ab52642 staining S100 beta in human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A-375 (human malignant melanoma cell line) cells labeling S100 beta with purified ab52642 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) ab150077 secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue). The negative control is as follows;
    ab52642 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

     

  • Immunohistochemical analysis of paraffin embedded human cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

  • All lanes : Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/10000 dilution (purified)

    Lane 1 : Mouse spinal cord
    Lane 2 : Rat brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

  • Immunocytochemistry/ Immunofluorescence analysis of mouse colon-derived neurospheres labeling S100 beta with ab52642 at 1/400 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Next the cells were blocked with 5 % serum for 1 hour at 25°C, followed by incubation with anti-S100 beta antibody [EP1576Y] (ab52642) in 1% BSA for 18 hours at 4°C. A polyclonal goat anti-rabbit IgG Alexa Fluor® 594 was used at 1/200 dilution.

    See Abreview

  • Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/5000 dilution (purified) + B16F0 (mouse melanoma cell line) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa

    Blocking  and Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin embedded mouse cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

  • S100 beta was immunoprecipitated from human fetal brain with purified ab52642 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52642 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

  • Immunohistochemical analysis of embryonic mouse brain tissue, staining S100 beta with unpurified ab52642.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

文献

This product has been referenced in:
  • Song J  et al. Biodegradable and biocompatible cationic polymer delivering microRNA-221/222 promotes nerve regeneration after sciatic nerve crush. Int J Nanomedicine 12:4195-4208 (2017). Read more (PubMed: 28652727) »
  • Kanao S  et al. Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells In Vitro. Stem Cells Int 2017:8371326 (2017). Read more (PubMed: 28261273) »

See all 49 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole Brain Tissue Lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-8% Tris-Acetate)
Loading amount
20 µg
Specification
Whole Brain Tissue Lysate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jun 22 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Whole Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Tween-20
Specification
Whole Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 29 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (muscle layer of the colon)
Specification
muscle layer of the colon
Username

Abcam user community

Verified customer

提交于 Feb 02 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (colon-derived neurospheres)
Permeabilization
Yes - 0.1% Triton X-100
Specification
colon-derived neurospheres
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 02 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (large intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization
Yes - 0.1% Triton X-100
Specification
large intestine
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 16 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (large intestine)
Permeabilization
Yes - 0.1% Triton X-100
Specification
large intestine
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 16 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Adipose)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization
No
Specification
Adipose
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered Formalin
Username

Abcam user community

Verified customer

提交于 Aug 20 2015

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Human Cell (Adipose tissue)
Specification
Adipose tissue
Permeabilization
Yes - 0.3%TritonX-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 16 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 26 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Sample
Human Tissue sections (colon cancer tissue specimen)
Specification
colon cancer tissue specimen
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 26 2015

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