Anti-S100 beta抗体[EP1576Y] (ab52642)

Anti-S100 beta antibody [EP1576Y] 图像

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] (ab52642)This image is courtesy of an Abreview submitted by Heiko Locher

  Unpurified ab52642 staining S100 beta in Human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/200 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor^® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody [EP1576Y] (ab52642)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A-375 (Human malignant melanoma cell line) cells labeling S100 beta with purified ab52642 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor^® 488) ab150077 secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue). The negative control is as follows;
  ab52642 at 1/100 dilution followed by ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.

   

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] (ab52642)

  Immunohistochemical analysis of paraffin embedded Human cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

  Negative control: Using PBS instead of primary antibody.

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  Western blot - Anti-S100 beta antibody [EP1576Y] (ab52642)
  All lanes : Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/10000 dilution (purified)
  
  Lane 1 : Mouse spinal cord
  Lane 2 : Rat brain
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 11 kDa
  Observed band size : 11 kDa
  

  Blocking and Diluting buffer and concentration: 5% NFDM/TBST

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] (ab52642)

  Immunohistochemical analysis of paraffin embedded Rat cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

  Negative control: Using PBS instead of primary antibody.

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  Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody [EP1576Y] (ab52642)This image is courtesy of an anonymous abreview.

  Immunocytochemistry/ Immunofluorescence analysis of mouse colon-derived neurospheres labeling S100 beta with ab52642 at 1/400 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Next the cells were blocked with 5 % serum for 1 hour at 25°C, followed by incubation with Anti-S100 beta antibody [EP1576Y] (ab52642) in 1% BSA for 18 hours at 4°C. A polyclonal goat anti-rabbit IgG Alexa Fluor^® 594 was used at 1/200 dilution.

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  Western blot - Anti-S100 beta antibody [EP1576Y] (ab52642)
  Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/5000 dilution (purified) + B16F0 (Mouse melanoma cell line) at 10 µg
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 11 kDa
  Observed band size : 11 kDa
  

  Blocking  and Diluting buffer and concentration: 5% NFDM/TBST

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] (ab52642)

  Immunohistochemical analysis of paraffin embedded Mouse cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

  Negative control: Using PBS instead of primary antibody.

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  Western blot - Anti-S100 beta antibody [EP1576Y] (ab52642)
  Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/5000 dilution (purified) + A-375 (Human malignant melanoma cell line) at 10 µg
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 11 kDa
  Observed band size : 11 kDa
  

  Blocking and Diluting buffer and concentration: 5% NFDM/TBST

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  Immunoprecipitation - Anti-S100 beta antibody [EP1576Y] (ab52642)

  S100 beta was immunoprecipitated from Human fetal brain with purified ab52642 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52642 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution.

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

   

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] (ab52642)Image from Selvadurai HJ & Mason JO, PLoS One. 2011;6(8):e23012. Epub 2011 Aug 8. Fig 5.; doi:10.1371/journal.pone.0023012; August 8, 2011, PLoS ONE 6(8): e23012.

  Immunohistochemical analysis of embryonic mouse brain tissue, staining S100 beta with unpurified ab52642.

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  Other - Anti-S100 beta antibody [EP1576Y] (ab52642)
  Equilibrium disassociation constant (K_D)
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