Anti-S100 beta抗体- Astrocyte Marker (ab41548)

概述

  • 产品名称
    Anti-S100 beta抗体- Astrocyte Marker
    参阅全部 S100 beta 一抗
  • 描述
    兔多克隆抗体to S100 beta - Astrocyte Marker
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-FoFr, IHC-Pmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Pig
    预测可用于: Rabbit, Cow, Human, Chinese hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Rat S100 beta.

    (Peptide available as ab41547.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates; Brain (Rat), Spinal Cord (Mouse), Spinal Cord (Rat)

性能

应用

Our Abpromise guarantee covers the use of ab41548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).Can be blocked with Rat S100 beta peptide (ab41547).
IHC-FoFr 1/3000.
IHC-P Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 功能
      Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
    • 组织特异性
      Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
    • 序列相似性
      Belongs to the S-101 family.
      Contains 2 EF-hand domains.
    • 细胞定位
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • NEF antibody
      • Protein S100 B antibody
      • Protein S100-B antibody
      • S 100 calcium binding protein beta chain antibody
      • S 100 protein beta chain antibody
      • S-100 protein beta chain antibody
      • S-100 protein subunit beta antibody
      • S100 antibody
      • S100 calcium binding protein beta (neural) antibody
      • S100 calcium-binding protein B antibody
      • S100 protein beta chain antibody
      • S100B antibody
      • S100B_HUMAN antibody
      • S100beta antibody
      see all

    图片

    • ab41548 staining S100 beta in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

      At this dilution factor, there is very good delineation of astrocyte processes.

      See Abreview

    • All lanes : Anti-S100 beta antibody - Astrocyte Marker (ab41548) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Spinal Cord (Mouse) Tissue Lysate
      Lane 4 : Spinal Cord (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 11 kDa
      Observed band size: 9 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.

    • ab41548 staining S100 beta in Zebrafish body, showing T/S of spinal cord region by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

      See Abreview

    • ab41548 staining S100 beta in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/3000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.

      See Abreview

    文献

    This product has been referenced in:
    • Li JJ  et al. In vivo evidence for the contribution of peripheral circulating inflammatory exosomes to neuroinflammation. J Neuroinflammation 15:8 (2018). IHC ; Mouse . Read more (PubMed: 29310666) »
    • Tatsumi K  et al. Olig2-Lineage Astrocytes: A Distinct Subtype of Astrocytes That Differs from GFAP Astrocytes. Front Neuroanat 12:8 (2018). ICC/IF . Read more (PubMed: 29497365) »

    See all 22 Publications for this product

    客户评价及客户问答

    Thank you very much for your inquiry. I am sorry to hear that the customer is having troubles with this antibody.

    I have looked through the protocol and through some available literature. Indeed, it seems that the S100 beta proteins can form ...

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