概述

  • 产品名称Anti-S100抗体[S100 217] (FITC)
    参阅全部 S100 一抗
  • 描述
    小鼠单克隆抗体[S100 217] to S100 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: Flow Cyt, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Monkey
  • 免疫原

    Full length native protein (purified) (Cow)

  • 常规说明This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis.

性能

应用

Our Abpromise guarantee covers the use of ab76749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.
ICC/IF Use a concentration of 10 - 20 µg/ml.

Incubate for 2 hours in the dark at RT. The incubation can also be left overnight at 4ºC.

靶标

  • 功能Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • 组织特异性Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • 序列相似性Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody [S100 217] (FITC) 图像

  • Overlay histogram showing SH-SY5Y cells stained with ab76749 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76749, 0.1μg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [MOPC-21] (FITC) (ab18435, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-S100 antibody [S100 217] (FITC) (ab76749)参考文献

This product has been referenced in:
  • Zhu X  et al. Schwann cell proliferation and differentiation that is induced by ferulic acid through MEK1/ERK1/2 signalling promotes peripheral nerve remyelination following crush injury in rats. Exp Ther Med 12:1915-1921 (2016). Rat . Read more (PubMed: 27588110) »

See 1 Publication for this product

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