Anti-S100抗体[B32.1] (ab7852)
Key features and details
- Mouse monoclonal [B32.1] to S100
- Suitable for: IHC-FoFr
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-S100抗体[B32.1]
参阅全部 S100 一抗 -
描述
小鼠单克隆抗体[B32.1] to S100 -
宿主
Mouse -
特异性
This antibody reacts with alpha and beta subunits of human S-100 protein (21-24 kD). -
经测试应用
适用于: IHC-FoFrmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Cow S100.
Database link: P02639 -
阳性对照
- Melanoma tissues containing nerves, such as intestine and pancreas. Brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
B32.1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
---|---|---|
IHC-FoFr |
1/500 - 1/1000. 1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4.
|
说明 |
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IHC-FoFr
1/500 - 1/1000. 1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4. |
靶标
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功能
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
组织特异性
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
序列相似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 6271 Human
- Omim: 176940 Human
- SwissProt: P23297 Human
- Unigene: 515715 Human
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别名
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
图片
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ab7852 at a dilution of 1/1000, staining Astrocytes (purple; Alexa 488 secondary at 1/2000) on 30
µ m coronal brain section tissue in free floating IHC (see protocol link for detailed description). [A] Image taken with 20x objective and [B] Image taken with 40x objective. Images coloured in Photoshop.NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab7852 被引用在 17 文献中.
- Galiakberova AA et al. IPSC-Derived Human Neurons with GCaMP6s Expression Allow In Vitro Study of Neurophysiological Responses to Neurochemicals. Neurochem Res 47:952-966 (2022). PubMed: 34855047
- Gomez-Sintes R et al. Targeting retinoic acid receptor alpha-corepressor interaction activates chaperone-mediated autophagy and protects against retinal degeneration. Nat Commun 13:4220 (2022). PubMed: 35864098
- Arotcarena ML et al. Pilot Study Assessing the Impact of Intrathecal Administration of Variants AAV-PHP.B and AAV-PHP.eB on Brain Transduction in Adult Rhesus Macaques. Front Bioeng Biotechnol 9:762209 (2021). PubMed: 34869273
- Alhindi A et al. Terminal Schwann cells at the human neuromuscular junction. Brain Commun 3:fcab081 (2021). PubMed: 33977269
- Wu P et al. Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair. Bioact Mater 6:3515-3527 (2021). PubMed: 33842738