Anti-S100抗体(ab34686)
Key features and details
- Rabbit polyclonal to S100
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-S100抗体
参阅全部 S100 一抗 -
描述
兔多克隆抗体to S100 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human
预测可用于: Dog, Non human primates, Mammals -
免疫原
Full length native protein (purified): MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKELLQT ELSGFLDAQK DADAVDKVMK ELDENGDGEV DFQEYVVLVA ALTVACNNFF WENS, corresponding to amino acids 1-94 of Cow S100 (alpha chain). A mixture of alpha alpha homodimers and alpha beta heterodimers was used.
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常规说明
Please note that lot 296545 is supplied at 0.5mg/ml.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
This antibody was purified from monospecific antiserum. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34686于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1.67 - 10 µg/ml. Detects a band of approximately 11 kDa.
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IHC-P | (4) |
Use a concentration of 2.5 - 25 µg/ml.
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说明 |
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WB
Use a concentration of 1.67 - 10 µg/ml. Detects a band of approximately 11 kDa. |
IHC-P
Use a concentration of 2.5 - 25 µg/ml. |
靶标
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功能
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
组织特异性
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
序列相似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 431787 Cow
- Entrez Gene: 6271 Human
- Entrez Gene: 20193 Mouse
- Entrez Gene: 295214 Rat
- Omim: 176940 Human
- SwissProt: P02639 Cow
- SwissProt: P23297 Human
- SwissProt: P56565 Mouse
see all -
别名
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
图片
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ab34686 staining S100 in Rat nerve tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a 10mM citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/250 in PBS + 1x casein) for 1 hour at 25°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
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All lanes : Anti-S100 antibody (ab34686) at 1/1000 dilution
Lane 1 : Bovine S100 monomer at 0.1 µg
Lane 2 : S100 from rat brain lysate at 35 µg
Secondary
All lanes : IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX
Observed band size: 11 kDa why is the actual band size different from the predicted? -
Ab34686, at a 1/500 dilution, staining S100 in mouse brain tissue by immunohistochemistry. The tissue was formalin fixed and embedded in paraffin. Dark nuclear staining is observed within basket cells located near the Purkinje cells in the cerebellum.
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Ab34686, at 1/500 dilution, staining S100 in formalin fixed and paraffin embedded human brain astrocyte tumor tissue by immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab34686 被引用在 7 文献中.
- Shibata O et al. Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method. Mol Ther Nucleic Acids 28:342-352 (2022). PubMed: 35474735
- Su Y et al. Small Intestinal Submucosa Biomimetic Periosteum Promotes Bone Regeneration. Membranes (Basel) 12:N/A (2022). PubMed: 35877922
- Kakuta W et al. Regeneration of sensory nerve branches in extraction socket and surrounding alveolar bone in rat: immunohistochemical observation of the axon and myelin sheath changes. Odontology N/A:N/A (2022). PubMed: 36460825
- Wai V et al. The Anatomical Distribution of Mechanoreceptors in Mouse Hind Paw Skin and the Influence of Integrin a1ß1 on Meissner-Like Corpuscle Density in the Footpads. Front Neuroanat 15:628711 (2021). PubMed: 33737870
- Sohn EJ et al. Involvement of the miR-363-5p/P2RX4 Axis in Regulating Schwann Cell Phenotype after Nerve Injury. Int J Mol Sci 22:N/A (2021). PubMed: 34769029
- Gao L et al. Targeted next-generation sequencing of dedifferentiated chondrosarcoma in the skull base reveals combined TP53 and PTEN mutations with increased proliferation index, an implication for pathogenesis. Oncotarget 7:43557-43569 (2016). PubMed: 27248819
- Hsu M & Stevenson FF Reduction in muscular motility by selective focused cold therapy: a preclinical study. J Neural Transm 121:15-20 (2014). IHC-P ; Rat . PubMed: 23917804