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Full length protein (Human).
Our Abpromise guarantee covers the use of ab8330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC - Wholemount||Use at an assay dependent concentration. PubMed: 21856924|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.|
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [6G1] to S100 (HRP) (ab10203). Sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5mM CaCL2 in these assay buffers. Can be used as capture antibody in conjunction with ab10203.|
ab8330 at a dilution of 1/1000, staining S100 (Alexa 488 secondary at 1/2000) on rat brain tissue (30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).