Anti-S100抗体[6G1] (HRP) (ab10203)

概述

  • 产品名称Anti-S100抗体[6G1] (HRP)
    参阅全部 S100 一抗
  • 描述
    小鼠单克隆抗体[6G1] to S100 (HRP)
  • 偶联物HRP
  • 特异性Clone 6G1 is specific for S-100 beta beta and and S100 alpha beta
  • 经测试应用适用于: ELISA, Sandwich ELISA, Purification, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human S-100 protein

  • 常规说明Concentration varies from lot to lot and can be provided on request.

性能

应用

Our Abpromise guarantee covers the use of ab10203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [8B10] to S100 (ab8330) and Mouse monoclonal [3B10] to S100 (ab8334). Use as Detection antibody. Sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5mM CaCl2 in thses assay buffers. Can be used as detection antibody in conjunction with ab8330 and ab8334.
Purification Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

靶标

  • 功能Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • 组织特异性Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • 序列相似性Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody [6G1] (HRP) 图像

  • S-100 calibration curve, from one step assay in streptavidin coated plates. Ab8330 [200 ng/well] was used as capture and Eu- labelled ab10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20°C.

Anti-S100 antibody [6G1] (HRP) (ab10203)参考文献

ab10203 has not yet been referenced specifically in any publications.

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I can confirm that the antibodies mentioned (ab8330 and a...

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