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Our Abpromise guarantee covers the use of ab4066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23624931|
|IHC-P||1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.|
|IHC-Fr||1/200 - 1/500.|
|WB||Use at an assay dependent concentration.|
ab4066 at a dilution of 1/200, staining S100 in astrocytes (Alexa 488 secondary at 1/2000) on 30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
ab4066 at a 1/100 dilution staining rat cortical preps grown 14 days in culture, plated onto coverslips. The preps were acid/alcohol fixed for 15 minutes, blocked and then incubated with the antibody for 1 hour. Bound antibody was detected using a Alexa fluor ® 555 conjugated goat polyclonal antibody. Nuclei were visualised using DAPI (blue). Note: s100 is colocalizing around the nucleus.
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