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Our Abpromise guarantee covers the use of ab14849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.|
|IHC-P||1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See Abreview.|
ab14849 staining S100 in Human melanoma cultured cells by ICC (Immunocytochemistry). Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.2% Triton X-100 for 15 minutes and blocked for 30 minutes at 21°C. Samples were incubated with primary antibody (at concentrations listed above) for 15 hours at 4°C. A HRP-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.
Interaction of several monoclonal antibodies with purified S100 protein (1