概述

  • 产品名称Anti-S100抗体[3B10]
    参阅全部 S100 一抗
  • 描述
    小鼠单克隆抗体[3B10] to S100
  • 特异性Specific to S-100 alpha beta
  • 经测试应用适用于: ELISA, WB, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (Human).

  • 阳性对照
    • Human brain.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab8334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [6G1] to S100 (HRP) (ab10203).
WB Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [6G1] to S100 (HRP) (ab10203). Sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5mM CaCl2 in these assay buffers. Can be used as capture antibody in conjunction with ab10203.

靶标

  • 功能Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • 组织特异性Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • 序列相似性Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody [3B10] 图像

  • Interaction of several monoclonal antibodies with purified S100 protein (1 µg) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334. The doublet band appears at about 20-21 kD, Interaction of several monoclonal antibodies with purified S100 protein (1 µg) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334. The doublet band appears at about 20-21 kD,

Anti-S100 antibody [3B10] (ab8334)参考文献

This product has been referenced in:
  • Siman R  et al. Evidence that a Panel of Neurodegeneration Biomarkers Predicts Vasospasm, Infarction, and Outcome in Aneurysmal Subarachnoid Hemorrhage. PLoS One 6:e28938 (2011). Sandwich ELISA ; Human . Read more (PubMed: 22174930) »

See 1 Publication for this product

Product Wall

Thank you for contacting us and sorry for the delay in getting back to you.

Kate is unfortunately still not in the office but I will try to answer your questions as best I can.

I can confirm that the antibodies mentioned (ab8330 and a...

Read More

Sorry, we have not yet done the cross-reactivity testing with S-100 protein coming from other species for our S-100 protein clones.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"