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Our Abpromise guarantee covers the use of ab8334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [6G1] to S100 (HRP) (ab10203).|
|WB||Use at an assay dependent dilution.|
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [6G1] to S100 (HRP) (ab10203). Sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5mM CaCl2 in these assay buffers. Can be used as capture antibody in conjunction with ab10203.|
Interaction of several monoclonal antibodies with purified S100 protein (1