概述

  • 产品名称Anti-S100 alpha抗体
    参阅全部 S100 alpha 一抗
  • 描述
    兔多克隆抗体to S100 alpha
  • 经测试应用适用于: ELISA, IP, WB, IHC-P, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Dog, Human, Pig
  • 免疫原

    Full length native protein (purified) corresponding to Human S100 alpha. Purified S100-alpha protein from human pectoral muscle cells.

  • 阳性对照
    • Human muscle.

性能

应用

Our Abpromise guarantee covers the use of ab11428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). Use at a concentration of 0.1 µg/ml. Detects a band of approximately 10 kDa, representing S100-alpha from human muscle. S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
IHC-P Use a concentration of 1 µg/ml.
ICC 1/50 - 1/200.
ICC/IF 1/50 - 1/200.

靶标

Anti-S100 alpha antibody 图像

  • ab11428 labelling S100 alpha (red) in HEK293T cells by Immunocytochemistry/Immunofluorescence. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1:100 in blocking buffer) overnight at 4ºC. A fluorophore-conjugated goat anti-rabbit IgG (1:200) was used as the secondary antibody (1 hour at room temperature). Blue (DAPI) - nuclei. Images were taken at 40X magnification.



  • Predicted band size : 10 kDa

    Figure 1 shows a Western blot of S100-alpha from human muscle extract using ab11428.

  • ab11428 (2µg/ml) staining S100 alpha in human breast tissue using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and cytoplasmic compartment within the breast ductal regions
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab11428 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11428, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-S100 alpha antibody (ab11428)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for your patience. I have heard back from the lab with the following information: We used 100ug human skeletal muscle lysate which is shown on our website and we followed a standard Western blotting procedure. It is recommended...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Tissue lysate - whole (Left ventricle)
Loading amount 3 µg
Specification Left ventricle
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. sudhish mishra

Verified customer

提交于 Apr 29 2008

I apologise for the delay in replying to you, the laboratory has reopened yesterday in the US because of a bank holiday on Monday. I can confirm that ab11428 has not been tested in renal carcinomas. This antibody has shown no cross-reactivity with...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Ovary)
Loading amount 50 µg
Specification Ovary
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. John Andersen

Verified customer

提交于 Jun 14 2006

Thank you for your email. Ab11177 has not yet been tested for application in WB or for cross-reactivity with mouse, but ab11428 has. Therefore, I would recommend ab11428 to you. We do not routinely offer free or trial sized samples for testing purp...

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Here is the reference to the paper that uses our antibody in IHC procedures: K. Kato, et al. Enzyme Immunoassay for Measurement of the alpha Subunit of S100 Protein in Human Biological Fluids. Biomed. Res., 8(2): 119-125, 1987. Unfortunate...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"