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Recombinant full length protein (see pET-32a-c(+) vector from Novagen)
Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab24838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ELISA||Use at an assay dependent concentration.|
|Purification||Use at an assay dependent concentration.|
ab24838 staining human HeLa cells by ICC/IF. Cells were PFA fixed and blocked with 3% BSA for 1 hour at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. A Cy2® conjugated donkey anti-mouse antibody, diluted 1/300 was used as the secondary.
The slide was stained with Dapi, S Tag-Cy2 and CREST-Cy3.