Anti-S-nitrosocysteine抗体[HY8E12] (ab94930)

概述

  • 产品名称Anti-S-nitrosocysteine抗体[HY8E12]
  • 描述
    小鼠单克隆抗体[HY8E12] to S-nitrosocysteine
  • 特异性ab94930 targets conjugated S-nitrosocysteine, it does not recognize free S-nitrosocysteine.
  • 经测试应用适用于: ICC, WB, IHC-P, IHC-Frmore details
  • 免疫原

    Chemical/ Small Molecule corresponding to S-nitrosocysteine conjugated to Bovine Serum Albumin (BSA).

性能

应用

Our Abpromise guarantee covers the use of ab94930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/1000 - 1/5000.

ab131369 can be used as blocking peptide.

WB 1/1000 - 1/2000.
IHC-P 1/1000 - 1/5000.

Fixation of tissue for use with this antibody should be done with glutaraldehyde. The use of paraformaldehyde in conjunction with glutaraldehyde may improve staining results.

ab131369 can be used as blocking peptide.

IHC-Fr Use at an assay dependent concentration.

ab131369 can be used as blocking peptide.

靶标

  • 相关性All mammalian cells contain low levels of nitrosylated proteins, that are thought to be regulated by Snitrosylation and denitrosylation. S nitrosylation of proteins serves as a ubiquitous post-translational modification that dynamically regulates a broad functional spectrum of proteins. The majority of these proteins are regulated by S nitrosylation on a single critical cysteine residue within an acidic/basic or hydrophobic structural motif, that may also be subject to oxygen or glutathione dependent modification, suggesting that S nitrosylation is a prototypic redox signal.

Anti-S-nitrosocysteine antibody [HY8E12] 图像

  • Image from Ding SY et al., J Biol Chem. 2011 May 13;286(19):16768-74. Epub 2011 Mar 25.; Fig 2.; doi: 10.1074/jbc.M110.192799; May 13, 2011 The Journal of Biological Chemistry, 286, 16768-16774.

    Mouse pancreatic islets were treated with 30 nm GLP-1 for 10 min prior to lysis. GCK was immunoprecipitated and assessed for S-nitrosocysteine by Western blotting using ab94930.

Anti-S-nitrosocysteine antibody [HY8E12] (ab94930)参考文献

This product has been referenced in:
  • Ding SY  et al. Glucagon-like peptide 1 stimulates post-translational activation of glucokinase in pancreatic beta cells. J Biol Chem 286:16768-74 (2011). WB . Read more (PubMed: 21454584) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount 30 µg
Specification Melanoma cancer
Treatment 5gy Irradiation
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

提交于 Feb 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"