概述

  • 产品名称
    (S)-5-Nitrowillardiine
  • 描述
    AMPA / kainate agonist
  • 生物学描述
    Broad spectrum agonist for AMPA and kainate receptors with no activity at NMDA or metabotropic receptors
  • 纯度
    > 98%

性能

应用

Our Abpromise guarantee covers the use of ab120063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

图片

  • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Nitrowillardiine (ab120063), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-5-Nitrowillardiine, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120063 ((S)-5-Nitrowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

文献

This product has been referenced in:
  • Jane DE  et al. Synthesis of willardiine and 6-azawillardiine analogs: pharmacological characterization on cloned homomeric human AMPA and kainate receptor subtypes. J Med Chem 40:3645-50 (1997). Read more (PubMed: 9357531) »
  • Wong LA  et al. Willardiines differentiate agonist binding sites for kainate- versus AMPA-preferring glutamate receptors in DRG and hippocampal neurons. J Neurosci 14:3881-97 (1994). Read more (PubMed: 7515954) »

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting Abcam regarding ab120063 (Asc-063).


I have had a quick look at the literature and haven't found any information regarding UV absorption of Nitrowillardiine.


We have a UV absorbance spectrum, obta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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