(S)-5-Nitrowillardiine,AMPA / kainate激动剂(ab120063)
Key features and details
- AMPA / kainate agonist
- CAS Number:
- Soluble in 1 eq. NaOH to 50 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
(S)-5-Nitrowillardiine,AMPA / kainate激动剂 -
描述
AMPA / kainate激动剂 -
生物学描述
Broad spectrum agonist for AMPA and kainate receptors with no activity at NMDA or metabotropic receptors
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化学结构
性能
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化学名称
(S)-2-Amino-3-(3,4-dihydro-5-nitro-2,4-dioxopyrimidin-1(2H)-yl)propanoic acid -
分子量
244.16 -
分子式
C7H8N4O6 -
PubChem识别号
11096831 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in 1 eq. NaOH to 50 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
N[C@@H](CN1C=C(C(=O)NC1=O)[N+]([O-])=O)C(=O)O -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120063于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Functional Studies |
Use at an assay dependent concentration.
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说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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2D chemical structure image of ab120063, (S)-5-Nitrowillardiine, AMPA / kainate agonist
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ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Nitrowillardiine (ab120063), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-5-Nitrowillardiine, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120063 ((S)-5-Nitrowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (1)
ab120063 被引用在 1 文献中.
- Poon K et al. Mechanisms of modal activation of GluA3 receptors. Mol Pharmacol 80:49-59 (2011). PubMed: 21464198