Anti-RYK抗体[EPR5169(2)] (ab124961)


  • 产品名称Anti-RYK抗体[EPR5169(2)]
    参阅全部 RYK 一抗
  • 描述
    兔单克隆抗体[EPR5169(2)] to RYK
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to residues in Human RYK (intracellular domain).

  • 阳性对照
    • 293T, MCF7 and Jurkat cell lysates; Human brain tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab124961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000 - 1/200000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.


  • 功能May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
  • 组织特异性Observed in all the tissues examined.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 protein kinase domain.
    Contains 1 WIF domain.
  • 结构域The extracellular WIF domain is responsible for Wnt binding.
  • 翻译后修饰Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation. Cleavage occurs during neuronal differentiation.
  • 细胞定位Membrane. Nucleus. Cytoplasm. In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D3S3195 antibody
    • ERK 3 antibody
    • Hydroxyaryl protein kinase antibody
    • JTK 5 antibody
    • JTK 5A antibody
    • JTK5 antibody
    • JTK5A antibody
    • JTK5A protein tyrosine kinase antibody
    • receptor like tyrosine kinase antibody
    • receptor like tyrosine kinase precursor antibody
    • RYK 1 antibody
    • Ryk antibody
    • RYK_HUMAN antibody
    • RYK1 antibody
    • Tyrosine protein kinase RYK antibody
    • Tyrosine protein kinase RYK precursor antibody
    • Tyrosine-protein kinase RYK antibody
    • Vik antibody
    see all

Anti-RYK antibody [EPR5169(2)] 图像

  • All lanes : Anti-RYK antibody [EPR5169(2)] (ab124961) at 1/50000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : MCF7 cell lysates
    Lane 3 : Jurkat cell lysates

    Lysates/proteins at 10 µg per lane.

    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 68 kDa
  • ab124961, at 1/50 dilution staining RYK in paraffin-embedded Human brain tissue, by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-RYK antibody [EPR5169(2)] (ab124961)参考文献

ab124961 has not yet been referenced specifically in any publications.

Product Wall

The antigen retrieval step is typically done after de-paraffinization and before any blocking steps. The aim is to partially reverse the effects of formaldehyde-based fixatives, to encourage recognition of the protein of interest by the antibody. The c...

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