Anti-Ryanodine Receptor抗体- Carboxyterminal end (ab55999)

概述

  • 产品名称Anti-Ryanodine Receptor抗体- Carboxyterminal end
    参阅全部 Ryanodine Receptor 一抗
  • 描述
    兔多克隆抗体to Ryanodine Receptor - Carboxyterminal end
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to C-terminal residues of human RYR1 (Ryanodine receptor 1)

性能

应用

Our Abpromise guarantee covers the use of ab55999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml for 2hrs. Predicted molecular weight: 559 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.
    • 组织特异性Skeletal muscle and brain (cerebellum and hippocampus).
    • 疾病相关Malignant hyperthermia 1
      Central core disease of muscle
      Multiminicore disease with external ophthalmoplegia
      Myopathy, congenital, with fiber-type disproportion
      Defects in RYR1 may be a cause of Samaritan myopathy, a congenital myopathy with benign course. Patients display severe hypotonia and respiratory distress at birth. Unlike other congenital myopathies, the health status constantly improves and patients are minimally affected at adulthood.
    • 序列相似性Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily.
      Contains 3 B30.2/SPRY domains.
      Contains 5 MIR domains.
    • 结构域The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum (SR) and the T-tubule.
    • 翻译后修饰Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843.
      Activated by reversible S-nitrosylation. Repeated very high-level exercise increases S-nitrosylation.
    • 细胞定位Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Arrhythmogenic right ventricular dysplasia 2 antibody
      • ARVC2 antibody
      • ARVD2 antibody
      • Brain ryanodine receptor calcium release channel antibody
      • Brain type ryanodine receptor antibody
      • Cardiac muscle ryanodine receptor calcium release channel antibody
      • Cardiac muscle type ryanodine receptor antibody
      • CCO antibody
      • Central core disease of muscle antibody
      • HBRR antibody
      • hRYR 2 antibody
      • hRYR2 antibody
      • MHS antibody
      • MHS1 antibody
      • Ryanodine receptor 1 (skeletal) antibody
      • Ryanodine receptor 1 antibody
      • Ryanodine receptor 2 (cardiac) antibody
      • Ryanodine receptor 2 antibody
      • Ryanodine receptor 3 antibody
      • Ryanodine receptor type1 antibody
      • RYDR antibody
      • RYR 1 antibody
      • RYR 2 antibody
      • RYR 3 antibody
      • RYR antibody
      • RYR-1 antibody
      • RyR1 antibody
      • RYR1_HUMAN antibody
      • RYR2 antibody
      • RYR3 antibody
      • Sarcoplasmic reticulum calcium release channel antibody
      • Skeletal muscle calcium release channel antibody
      • Skeletal muscle ryanodine receptor antibody
      • Skeletal muscle type ryanodine receptor antibody
      • Skeletal muscle-type ryanodine receptor antibody
      • SKRR antibody
      • Type 1 like ryanodine receptor antibody
      • Type 1 ryanodine receptor antibody
      • VTSIP antibody
      see all

    Anti-Ryanodine Receptor antibody - Carboxyterminal end (ab55999)参考文献

    ab55999 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (huh-7)
    Loading amount 20 µg
    Specification huh-7
    Treatment cells expressing viral protein
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 04 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"