Anti-Ryanodine receptor 2 (phospho S2808)抗体(ab59225)

概述

  • 产品名称Anti-Ryanodine receptor 2 (phospho S2808)抗体
    参阅全部 Ryanodine receptor 2 一抗
  • 描述
    兔多克隆抗体to Ryanodine receptor 2 (phospho S2808)
  • 特异性ab59225 detects endogenous levels of Ryanodine Receptor 2 only when phosphorylated at serine 2808.
  • 经测试应用适用于: IHC-P, ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Ryanodine receptor 2. Synthetic phosphopeptide derived from human Ryanodine Receptor around the phosphorylation site of serine 2808 (R-I-SP-Q-T).
    Database link: Q92736

  • 阳性对照
    • Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab59225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ELISA 1/5000.
ICC/IF Use at an assay dependent concentration. PubMed: 23173055
WB Use at an assay dependent concentration. PubMed: 21156754

靶标

  • 功能Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.
  • 组织特异性Detected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta.
  • 疾病相关Familial arrhythmogenic right ventricular dysplasia 2
    Ventricular tachycardia, catecholaminergic polymorphic 1, with or without atrial dysfunction and/or dilated cardiomyopathy
  • 序列相似性Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR2 subfamily.
    Contains 3 B30.2/SPRY domains.
    Contains 5 MIR domains.
  • 发展阶段Expressed in myometrium during pregnancy.
  • 结构域The calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm.
  • 翻译后修饰Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels.
    Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium.
  • 细胞定位Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARVC2 antibody
    • ARVD2 antibody
    • Cardiac muscle ryanodine receptor antibody
    • Cardiac muscle ryanodine receptor-calcium release channel antibody
    • hRYR-2 antibody
    • ryanodine receptor 2 (cardiac) antibody
    • Ryanodine receptor 2 antibody
    • RyR antibody
    • RYR-2 antibody
    • RyR2 antibody
    • RYR2_HUMAN antibody
    • Type 2 ryanodine receptor antibody
    • VTSIP antibody
    see all

Anti-Ryanodine receptor 2 (phospho S2808) antibody 图像

  • ab59225 (1/50 dilution) staining Ryanodine Receptor 2 in human brain tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunizing peptide.

    ab59225 (1/50 dilution) staining Ryanodine Receptor in human brain tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunizing peptide.

Anti-Ryanodine receptor 2 (phospho S2808) antibody (ab59225)参考文献

This product has been referenced in:
  • Beca S  et al. Phosphodiesterase type 3A regulates basal myocardial contractility through interacting with sarcoplasmic reticulum calcium ATPase type 2a signaling complexes in mouse heart. Circ Res 112:289-97 (2013). Read more (PubMed: 23168336) »
  • Huang Y  et al. Sarcoplasmic phospholamban protein is involved in the mechanisms of postresuscitation myocardial dysfunction and the cardioprotective effect of nitrite during resuscitation. PLoS One 8:e82552 (2013). WB ; Rat . Read more (PubMed: 24386101) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization Yes - 0.01% Triton X-100
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 26 2016

Thank you for your reply.


We have a number of phospho specific antibodies in our catalog, but ab59225 is the only phospho specific antibody we have to Ryanodine Receptors.


In order to determine specifically which residue...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"