Anti-Ryanodine receptor 2 (phospho S2808)抗体(ab59225)

概述

  • 产品名称
    Anti-Ryanodine receptor 2 (phospho S2808)抗体
    参阅全部 Ryanodine receptor 2 一抗
  • 描述
    兔多克隆抗体to Ryanodine receptor 2 (phospho S2808)
  • 特异性
    ab59225 detects endogenous levels of Ryanodine Receptor 2 only when phosphorylated at serine 2808.
  • 经测试应用
    适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Ryanodine receptor 2. Synthetic phosphopeptide derived from human Ryanodine Receptor around the phosphorylation site of serine 2808 (R-I-SP-Q-T).
    Database link: Q92736

  • 阳性对照
    • Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab59225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ELISA 1/5000.
WB Use at an assay dependent concentration. PubMed: 21156754

靶标

  • 功能
    Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.
  • 组织特异性
    Detected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta.
  • 疾病相关
    Familial arrhythmogenic right ventricular dysplasia 2
    Ventricular tachycardia, catecholaminergic polymorphic 1, with or without atrial dysfunction and/or dilated cardiomyopathy
  • 序列相似性
    Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR2 subfamily.
    Contains 3 B30.2/SPRY domains.
    Contains 5 MIR domains.
  • 发展阶段
    Expressed in myometrium during pregnancy.
  • 结构域
    The calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm.
  • 翻译后修饰
    Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels.
    Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium.
  • 细胞定位
    Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARVC2 antibody
    • ARVD2 antibody
    • Cardiac muscle ryanodine receptor antibody
    • Cardiac muscle ryanodine receptor-calcium release channel antibody
    • hRYR-2 antibody
    • ryanodine receptor 2 (cardiac) antibody
    • Ryanodine receptor 2 antibody
    • RyR antibody
    • RYR-2 antibody
    • RyR2 antibody
    • RYR2_HUMAN antibody
    • Type 2 ryanodine receptor antibody
    • VTSIP antibody
    see all

Anti-Ryanodine receptor 2 (phospho S2808) antibody 图像

  • ab59225 (1/50 dilution) staining Ryanodine Receptor 2 in human brain tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunizing peptide.

Anti-Ryanodine receptor 2 (phospho S2808) antibody (ab59225)参考文献

This product has been referenced in:
  • Park SJ  et al. Specific Sirt1 Activator-mediated Improvement in Glucose Homeostasis Requires Sirt1-Independent Activation of AMPK. EBioMedicine 18:128-138 (2017). WB . Read more (PubMed: 28396013) »
  • Castillero E  et al. Attenuation of the unfolded protein response and endoplasmic reticulum stress after mechanical unloading in dilated cardiomyopathy. Am J Physiol Heart Circ Physiol 309:H459-70 (2015). WB ; Human . Read more (PubMed: 26055788) »

See all 5 Publications for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - 0.01% Triton X-100
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 26 2016

Thank you for your reply.


We have a number of phospho specific antibodies in our catalog, but ab59225 is the only phospho specific antibody we have to Ryanodine Receptors.


In order to determine specifically which residue...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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