Anti-Ryanodine receptor 2抗体(ab117840)


  • 产品名称Anti-Ryanodine receptor 2抗体
    参阅全部 Ryanodine receptor 2 一抗
  • 描述
    兔多克隆抗体to Ryanodine receptor 2
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 2650 - 2750 of Human Ryanodine Receptor 2.

  • 阳性对照
    • This antibody gave a positive signal in Human heart formalin-fixed, paraffin-embedded tissue sections.



Our Abpromise guarantee covers the use of ab117840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • 功能Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.
  • 组织特异性Detected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta.
  • 疾病相关Familial arrhythmogenic right ventricular dysplasia 2
    Ventricular tachycardia, catecholaminergic polymorphic 1, with or without atrial dysfunction and/or dilated cardiomyopathy
  • 序列相似性Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR2 subfamily.
    Contains 3 B30.2/SPRY domains.
    Contains 5 MIR domains.
  • 发展阶段Expressed in myometrium during pregnancy.
  • 结构域The calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm.
  • 翻译后修饰Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels.
    Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium.
  • 细胞定位Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARVC2 antibody
    • ARVD2 antibody
    • Cardiac muscle ryanodine receptor antibody
    • Cardiac muscle ryanodine receptor-calcium release channel antibody
    • hRYR-2 antibody
    • ryanodine receptor 2 (cardiac) antibody
    • Ryanodine receptor 2 antibody
    • RyR antibody
    • RYR-2 antibody
    • RyR2 antibody
    • RYR2_HUMAN antibody
    • Type 2 ryanodine receptor antibody
    • VTSIP antibody
    see all

Anti-Ryanodine receptor 2 antibody 图像

  • IHC image of Ryanodine Receptor 2 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Ryanodine receptor 2 antibody (ab117840)参考文献

ab117840 has not yet been referenced specifically in any publications.

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