概述

  • 产品名称Anti-RuvB抗体
    参阅全部 RuvB 一抗
  • 描述
    小鼠多克隆抗体to RuvB
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Caenorhabditis elegans
  • 免疫原

    Recombinant fusion protein (Tagged): GLGDKAPHGI PPEMLDRLMI IPTMKYNEED IRKILVHRTE AENVQFEEKA FDLLTRLCAQ TCGREVIEVE DVDRCTKLFM DRGESLKKAE EEMRQPKNKK , corresponding to amino acids 359-458 of Caenorhabditis elegans RuvB

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 50 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性In Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity. In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciform structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC,RuvAB is uncoupled from RuvC and DSBs may be prevented.
  • 细胞定位Cell Membrane, Cytoplasmic and Nuclear
  • 数据库链接
    • 别名
      • ATP dependent DNA helicase, component of RuvABC resolvasome antibody
      • Holliday junction ATP dependent DNA helicase ruvB antibody
      • Holliday junction helicase subunit A antibody
      • RUVB (recombination protein) homolog antibody

    Anti-RuvB antibody 图像

    • All lanes : Anti-RuvB antibody (ab52783) at 1/1000 dilution

      Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a Tagged irrelevant antigen (Negative control)
      Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (Tagged fusion protein)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase at 1/5000 dilution

      Predicted band size : 50 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    Anti-RuvB antibody (ab52783)参考文献

    ab52783 has not yet been referenced specifically in any publications.

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