Anti-RuvA抗体(ab18576)

概述

• 产品名称Anti-RuvA抗体
  参阅全部 RuvA 一抗
• 描述
  兔多克隆抗体to RuvA
• 经测试应用适用于: WB, IPmore details
• 种属反应性
  与反应: Escherichia coli
  
• 免疫原

  Full length native protein (purified) (E. coli).

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• 纯度Whole antiserum
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Homologous Recomb.

相关产品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

应用

靶标

• 相关性In Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity. In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciform structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC, RuvAB is uncoupled from RuvC and DSBs may be prevented.
• 数据库链接
  • Entrez Gene: 5586077 Escherichia coli
  • Entrez Gene: 5595464 Escherichia coli
  • Entrez Gene: 913515 Escherichia coli
  • SwissProt: A7ZMY5 Escherichia coli
  • SwissProt: P0A809 Escherichia coli
• 别名
  • Holliday junction ATP dependent DNA helicase ruvA antibody
  • Holliday junction DNA helicase RuvA antibody
  • Ruv A antibody