Anti-RUNX3抗体- ChIP Grade (ab11905)

概述

  • 产品名称Anti-RUNX3抗体- ChIP Grade
    参阅全部 RUNX3 一抗
  • 描述
    兔多克隆抗体to RUNX3 - ChIP Grade
  • 经测试应用适用于: ChIP, WB, EMSA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • Raji nuclear extract. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A431

性能

应用

Our Abpromise guarantee covers the use of ab11905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 20714105
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
EMSA Use at an assay dependent concentration.
ICC/IF 1/200.

靶标

  • 功能CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
  • 序列相似性Contains 1 Runt domain.
  • 结构域A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
  • 翻译后修饰Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
  • 细胞定位Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acute myeloid leukemia 2 protein antibody
    • Acute myeloid leukemia gene 2 antibody
    • AML 2 antibody
    • AML2 antibody
    • CBF alpha 3 antibody
    • CBF-alpha-3 antibody
    • CBFA 3 antibody
    • CBFA3 antibody
    • Core binding factor alpha 3 subunit antibody
    • core binding factor antibody
    • Core binding factor runt domain alpha subunit 3 antibody
    • Core binding factor subunit alpha 3 antibody
    • core-binding factor antibody
    • Core-binding factor subunit alpha-3 antibody
    • Oncogene AML 2 antibody
    • Oncogene AML-2 antibody
    • PEA2 alpha C antibody
    • PEA2-alpha C antibody
    • PEBP2 alpha C antibody
    • PEBP2-alpha C antibody
    • Pebp2a3 antibody
    • PEBP2aC antibody
    • Polyomavirus enhancer binding protein 2 alpha C subunit antibody
    • Polyomavirus enhancer-binding protein 2 alpha C subunit antibody
    • runt domain alpha subunit 3 antibody
    • runt related transcription factor 3 antibody
    • Runt-related transcription factor 3 antibody
    • RUNX 3 antibody
    • Runx3 antibody
    • RUNX3_HUMAN antibody
    • SL3 3 enhancer factor 1 alpha C subunit antibody
    • SL3-3 enhancer factor 1 alpha C subunit antibody
    • SL3/AKV core binding factor alpha C subunit antibody
    • SL3/AKV core-binding factor alpha C subunit antibody
    • Transcription factor AML2 antibody
    see all

Anti-RUNX3 antibody - ChIP Grade 图像

  • Detection of AML2 by EMSA analysis. Nushift polyclonal antibody against AML2 factor creates a supershift in EMSA (lane 4).
  • ICC/IF image of ab11905 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11905 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RUNX3 antibody - ChIP Grade (ab11905)参考文献

This product has been referenced in:
  • Lai CB & Mager DL Role of Runt-related Transcription Factor 3 (RUNX3) in Transcription Regulation of Natural Cytotoxicity Receptor 1 (NCR1/NKp46), an Activating Natural Killer (NK) Cell Receptor. J Biol Chem 287:7324-34 (2012). WB, ChIP, EMSA ; Human . Read more (PubMed: 22253448) »
  • Ciavatta DJ  et al. Epigenetic basis for aberrant upregulation of autoantigen genes in humans with ANCA vasculitis. J Clin Invest 120:3209-19 (2010). WB, ChIP ; Human . Read more (PubMed: 20714105) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (RAJI cell lysate and HEK293T oveexpressing RUNX3)
Gel Running Conditions Reduced Denaturing (10% SDS gel)
Loading amount 35 µg
Treatment RUNX3 plasmid ytransfection for overexpresssion in 293T cells
Specification RAJI cell lysate and HEK293T oveexpressing RUNX3
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Yupen Chung

Verified customer

提交于 Apr 25 2016

Thank you for your patience with this inquiry. I am very sorry for the delay.
We sourced this particular product externally and regrettably the laboratory cannot provide the information, which isoform of the RUNX3 was used to produce this antibody....

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I am very pleased to hear you would like to accept our offer and test ab11905 in mouse. This code will give you xxx off your next order before ...

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Thank you for taking the time to submit these comments. It looks to me from the gel that the gel has not run out properly. Could you please enquire with the customer as to how the gel was made and how long the running gel and stacking gel were left to ...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab11905. For Western blotting, we do suggest using this antibody at 1:500 - 1:1000 and so I do suggest trying ab11905 at 1:500 with incubation overnight at 4C. W...

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