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Recombinant fragment: NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA, corresponding to amino acids 251-351 of Human RUNX2 (NP_004339) with tag.
Our Abpromise guarantee covers the use of ab76956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. Detection limit for recombinant tagged RUNX2 is approximately 0.1ng/ml when used as a capture antibody.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 21159735|
|Flow Cyt||Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
ab76956 staining RUNX2 in Mouse dveloping endochondral bone tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton and blocked with 10% Roche Western Blocking for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in blocking solution) for 16 hours. An undiluted Alexa Fluor® 555-conjugated Donkey polyclonal was used as the secondary antibody.