概述
- 产品名称Anti-RUNX1 / AML1抗体
参阅全部 RUNX1 / AML1 一抗 - 描述兔多克隆抗体to RUNX1 / AML1
- 经测试应用适用于: ICC/IF, WBmore details
- 种属反应性与反应: Mouse, Human
预测可用于: Rat, Cow, Dog
- 免疫原
Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (SLNHSTAFNP QPQSQMQDTR QIQPSPPWSY DQSYQYLGSI ASPSVHPATP) of Human RUNX1/ AML1 (NP_001745).
- 阳性对照
- SH-SYSY cell lysate
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab91002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
靶标
- 功能CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
- 组织特异性Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
- 疾病相关Note=A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.
Note=A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3'-end of TEL to the alternate 5'-exon of AML-1H.
Note=A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.
Defects in RUNX1 are the cause of familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]. FPDMM is an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
Note=A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16. - 序列相似性Contains 1 Runt domain.
- 结构域A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
- 翻译后修饰Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with MYST3.
Methylated. - 细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 529631 Cow
- Entrez Gene: 861 Human
- Entrez Gene: 12394 Mouse
- Entrez Gene: 50662 Rat
- Omim: 151385 Human
- SwissProt: Q01196 Human
- SwissProt: Q03347 Mouse
- SwissProt: Q63046 Rat
see all -
别名
- Acute myeloid leukemia 1 antibody
- Acute myeloid leukemia 1 protein antibody
- alpha subunit core binding factor antibody
see all
Anti-RUNX1 / AML1 antibody 图像
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Western blot - RUNX1 / AML1 antibody (ab91002)Anti-RUNX1 / AML1 antibody (ab91002) at 1 µg/ml (in 5% skim milk / PBS buffer) + SH-SYSY cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 52 kDa -
Immunocytochemistry/ Immunofluorescence-Anti-RUNX1 / AML1 antibody(ab91002)Immunofluorescence analysis of Mouse embryonic spinal motor neurons staining RUNX1 / AML1 with ab91002 at 1 µg/ml.
Anti-RUNX1 / AML1 antibody (ab91002)参考文献
ab91002 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"