Anti-RUNX1 / AML1抗体(ab35962)

概述

  • 产品名称Anti-RUNX1 / AML1抗体
    参阅全部 RUNX1 / AML1 一抗
  • 描述
    兔多克隆抗体to RUNX1 / AML1
  • 经测试应用适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human RUNX1/ AML1.

    (Peptide available as ab38695.)

  • 阳性对照
    • This antibody gave a positive signal in Human Jurkat nuclear and MOLT 4 lysates.

性能

应用

Our Abpromise guarantee covers the use of ab35962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/500.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 49 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
  • 组织特异性Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
  • 疾病相关Note=A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.
    Note=A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3'-end of TEL to the alternate 5'-exon of AML-1H.
    Note=A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.
    Defects in RUNX1 are the cause of familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]. FPDMM is an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
    Note=A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16.
  • 序列相似性Contains 1 Runt domain.
  • 结构域A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
  • 翻译后修饰Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with MYST3.
    Methylated.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acute myeloid leukemia 1 antibody
    • Acute myeloid leukemia 1 protein antibody
    • alpha subunit core binding factor antibody
    • AML 1 antibody
    • AML1 antibody
    • AML1 EVI 1 antibody
    • AML1 EVI 1 fusion protein antibody
    • Aml1 oncogene antibody
    • AMLCR 1 antibody
    • AMLCR1 antibody
    • CBF alpha 2 antibody
    • CBF-alpha-2 antibody
    • CBFA 2 antibody
    • CBFA2 antibody
    • Core binding factor alpha 2 subunit antibody
    • Core binding factor runt domain alpha subunit 2 antibody
    • Core-binding factor subunit alpha-2 antibody
    • EVI 1 antibody
    • EVI1 antibody
    • HGNC antibody
    • Oncogene AML 1 antibody
    • Oncogene AML-1 antibody
    • OTTHUMP00000108696 antibody
    • OTTHUMP00000108697 antibody
    • OTTHUMP00000108699 antibody
    • OTTHUMP00000108700 antibody
    • OTTHUMP00000108702 antibody
    • PEA2 alpha B antibody
    • PEA2-alpha B antibody
    • PEBP2 alpha B antibody
    • PEBP2-alpha B antibody
    • PEBP2A2 antibody
    • PEBP2aB antibody
    • Polyomavirus enhancer binding protein 2 alpha B subunit antibody
    • Polyomavirus enhancer-binding protein 2 alpha B subunit antibody
    • Run1 antibody
    • Runt related transcription factor 1 antibody
    • Runt-related transcription factor 1 antibody
    • RUNX 1 antibody
    • Runx1 antibody
    • RUNX1_HUMAN antibody
    • SL3 3 enhancer factor 1 alpha B subunit antibody
    • SL3-3 enhancer factor 1 alpha B subunit antibody
    • SL3/AKV core binding factor alpha B subunit antibody
    • SL3/AKV core-binding factor alpha B subunit antibody
    see all

Anti-RUNX1 / AML1 antibody 图像


  • Developed using the ECL technique

    Predicted band size : 49 kDa


    Exposure time : 1 minute

    RUNX2 recombinant protein full length, with N-terminal HIS tag, expressed in E.Coli.

    RUNX3 overexpression and empty vector control lysates created in HEK293T cells. The protein contains a C-terminal DDK tag.

  • All lanes : Anti-RUNX1 / AML1 antibody (ab35962) at 1 µg/ml

    Lane 1 : Jurkat nuclear extract lysate (ab14844)
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 52,54,55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds
  • ab35962 staining RUNX1/AML1 in Mouse Thymus tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 5 minutes of peroxidase block then 10 minutes of protein block. Antigen retrieval was by heat mediation in Retrieval solution. Samples were incubated with primary antibody (1/2000) for 45 minutes at 20°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.

    See Abreview

Anti-RUNX1 / AML1 antibody (ab35962)参考文献

This product has been referenced in:
  • Tsunoda T  et al. Immune-related zinc finger gene ZFAT is an essential transcriptional regulator for hematopoietic differentiation in blood islands. Proc Natl Acad Sci U S A 107:14199-204 (2010). IHC-P ; Mouse . Read more (PubMed: 20660741) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Primary osteoblast cells)
Total protein in input 300 µg
Specification Primary osteoblast cells
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

提交于 Dec 20 2012

Hello!

You must enroll in the AbTrial program before purchasing the product. Currently this product is out of stock and there is a note on our website recommending use of ab23980 as a replacment, as we cannot guarantee shipment of ab35962 in t...

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Thank you for calling Abcam yesterday.

The immunogen used to generate ab35962 was the one that was between aa440-453, which based on the sequence alignments you were doing, would not recognize all of the isoforms that the customer was interest...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (bladder, lung, liver, thymus)
Loading amount 20 µg
Specification bladder, lung, liver, thymus
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Megan Crow

Verified customer

提交于 Jul 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (thymus)
Specification thymus
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Jan 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"