概述

  • 产品名称Anti-RUBISCO抗体
  • 描述
    鸡多克隆抗体to RUBISCO
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Spinacia oleracea
    预测可用于: Plants
  • 免疫原

    Full length protein; RUBISCO small subunit (Spinach). NCBI Acc : AAC29137.1, Swiss-Prot Q9TIM3.

  • 阳性对照
    • Purified spinach RUBISCO.

性能

应用

Our Abpromise guarantee covers the use of ab62391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/2000. Detects bands of approximately 15 and 45 kDa (predicted molecular weight of precursor: 55 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
    • 序列相似性Belongs to the RuBisCO large chain family. Type I subfamily.
    • 翻译后修饰The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.
    • 细胞定位Plastid > chloroplast.
    • Information by UniProt
    • 数据库链接
      • 别名
        • 3 phospho D glycerate carboxylase antibody
        • rbcL antibody
        • RBL_SPIOL antibody
        • Ribulose 1,5 bisphosphate carboxylase/oxygenase small subunit N methyltransferase I antibody
        • Ribulose bisphosphate carboxylase large chain antibody
        • RuBisCO large subunit antibody
        see all

      Anti-RUBISCO antibody 图像

      • Anti-RUBISCO antibody (ab62391) at 1/2000 dilution + Purified spinach RUBISCO protein at 1 µg

        Secondary
        Goat anti-IgY-HRP at 1/1000 dilution

        Predicted band size : 55 kDa
        Observed band size : 15,45 kDa (why is the actual band size different from the predicted?)

      Anti-RUBISCO antibody (ab62391)参考文献

      This product has been referenced in:
      • Cai Z  et al. Development of an activity-directed selection system enabled significant improvement of the carboxylation efficiency of Rubisco. Protein Cell 5:552-62 (2014). WB . Read more (PubMed: 24870149) »

      See 1 Publication for this product

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"