概述

  • 产品名称
    Anti-Rubicon抗体
    参阅全部 Rubicon 一抗
  • 描述
    兔多克隆抗体to Rubicon
  • 经测试应用
    适用于: ICC/IF, WB, IP, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, from a region within amino acids 922-972 of Human Rubicon (NP_055502.1)

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab92388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-Fr Use at an assay dependent concentration. PubMed: 22843690

靶标

  • Information by UniProt
  • 数据库链接
  • 别名
    • Baron antibody
    • Beclin 1 associated RUN domain containing protein antibody
    • Beclin-1 associated RUN domain containing protein antibody
    • hypothetical protein LOC9711 antibody
    • Kiaa0226 antibody
    • OTTHUMP00000208021 antibody
    • OTTHUMP00000208023 antibody
    • OTTHUMP00000208024 antibody
    • RUBIC_HUMAN antibody
    • Rubicon antibody
    • RUN domain and cysteine rich domain containing, Beclin 1 interacting protein antibody
    • Run domain Beclin 1 interacting and cystein rich containing protein antibody
    • Run domain Beclin-1 interacting and cysteine-rich containing protein antibody
    • RUN domain protein as Beclin 1 interacting and cysteine rich containing antibody
    see all

图片

  • All lanes : Anti-Rubicon antibody (ab92388) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 109 kDa


    Exposure time : 3 minutes
  • Sample: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
    Antibodies: ab92388 used for WB 1 µg/ml (lane 1) and used for IP at 3 µg/mg lysate. Lane 2: control IgG.
    Detection: chemiluminescence with exposure time of 30 seconds.
  • ICC/IF image of ab92388 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92388, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of Human renal cancer tissue, staining Rubicon with ab92388. Tissue was fixed with acetone and incubated with primary antibody. A Cy3-conjugated anti-rabbit IgG was used as the secondary antibody.
  • Immunohistochemical analysis of Human renal tumour cells, staining Rubicon with ab92388. Cells were fixed with acetone and incubated with primary antibody. A Cy3-conjugated anti-rabbit IgG was used as the secondary antibody.

文献

This product has been referenced in:
  • Sun T  et al. Acetylation of Beclin 1 inhibits autophagosome maturation and promotes tumour growth. Nat Commun 6:7215 (2015). Read more (PubMed: 26008601) »
  • Bruntz RC  et al. Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells. J Biol Chem 289:600-16 (2014). WB ; Human . Read more (PubMed: 24257753) »

See all 3 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Microglia/astrocyte)
Permeabilization
Yes - 0.3% Troton X-100
Specification
Microglia/astrocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 11 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kiney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kiney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 20 2016

Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kiney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kiney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 02 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 02 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% triton x-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 01 2016

Unfortunately based on sequence alignment of the immunogen against zebrafish Rubicon, AB92388 does not qualify for the AbTrial program. This antibody has only 59% homology where we require 85% or higher.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册