概述

  • 产品名称Anti-RTF1抗体
    参阅全部 RTF1 一抗
  • 描述
    兔多克隆抗体to RTF1
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis , Medaka fish
  • 免疫原

    Immunogen corresponding to C terminal amino acids within Human RTF1 (EAW92492.1).

  • 阳性对照
    • HeLa whole cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab99362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Plays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA.
  • 序列相似性Contains 1 Plus3 domain.
  • 结构域The Plus3 domain mediates single-stranded DNA-binding.
  • 细胞定位Nucleus > nucleoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDG1N antibody
    • GTL 7 antibody
    • GTL7 antibody
    • KIAA0252 antibody
    • Ortholog of mouse gene trap locus 7 antibody
    • RNA polymerase associated protein RTF1 homolog antibody
    • RNA polymerase-associated protein RTF1 homolog antibody
    • RTF 1 antibody
    • RTF1 antibody
    • Rtf1 Paf1/RNA polymerase II complex component homolog antibody
    • RTF1_HUMAN antibody
    • rtf1L antibody
    • YGL244W antibody
    see all

Anti-RTF1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and mouse teratoma (right) tissues labelling RTF1 with ab99362 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-RTF1 antibody (ab99362) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 77 kDa


    Exposure time : 10 seconds
  • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99362 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: RTF1 antibody (ab99362) at 1 µg/ml

    developed using the ECL technique

    Exposure time : 3 seconds

    Predicted band size : 77 kDa

Anti-RTF1 antibody (ab99362)参考文献

ab99362 has not yet been referenced specifically in any publications.

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