概述

  • 产品名称Anti-RTF1抗体
    参阅全部 RTF1 一抗
  • 描述
    兔多克隆抗体to RTF1
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 300 and 350 of human RTF1 (EAW92492.1).

  • 阳性对照
    • HeLa Whole Cell Lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab70690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Plays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA.
    • 序列相似性Contains 1 Plus3 domain.
    • 结构域The Plus3 domain mediates single-stranded DNA-binding.
    • 细胞定位Nucleus > nucleoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CDG1N antibody
      • GTL 7 antibody
      • GTL7 antibody
      • KIAA0252 antibody
      • Ortholog of mouse gene trap locus 7 antibody
      • RNA polymerase associated protein RTF1 homolog antibody
      • RNA polymerase-associated protein RTF1 homolog antibody
      • RTF 1 antibody
      • RTF1 antibody
      • Rtf1 Paf1/RNA polymerase II complex component homolog antibody
      • RTF1_HUMAN antibody
      • rtf1L antibody
      • YGL244W antibody
      see all

    Anti-RTF1 antibody 图像

    • All lanes : Anti-RTF1 antibody (ab70690) at 0.4 µg/ml

      Lane 1 : Hela whole cell lysate at 50 µg
      Lane 2 : Hela whole cell lysate at 15 µg
      Lane 3 : Hela whole cell lysate at 5 µg


      Predicted band size : 77 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    Anti-RTF1 antibody (ab70690)参考文献

    This product has been referenced in:
    • Hoedt E  et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . Read more (PubMed: 25116916) »
    • Langenbacher AD  et al. The PAF1 complex differentially regulates cardiomyocyte specification. Dev Biol 353:19-28 (2011). WB ; Zebrafish . Read more (PubMed: 21338598) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (10% acrylamide gel)
    Sample Human Tissue lysate - whole (Brain)
    Specification Brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

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    Verified customer

    提交于 Feb 14 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"