Anti-RT1-Ba抗体[MRC OX-3] (Phycoerythrin) (ab123125)

概述

  • 产品名称Anti-RT1-Ba抗体[MRC OX-3] (Phycoerythrin)
    参阅全部 RT1-Ba 一抗
  • 描述
    小鼠单克隆抗体[MRC OX-3] to RT1-Ba (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性ab123125 recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, ACI, DA, or PVG/c strains. This antibody also cross-reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9. ab123125 recognizes Ia antigens on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes. Thus, ab123125 is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Rat thymocyte membrane glycoprotein

  • 阳性对照
    • Rat Strain: Buffalo (Thymus, Spleen, Lymph Node)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号MRC OX-3
  • 骨髓瘤P3-NS1/1-Ag4-1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab123125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.05µg for 106 cells.

靶标

  • 细胞定位Integral to membrane
  • 数据库链接
  • 别名
    • MHC class II antigen RT1.B alpha chain antibody
    • Rano class II histocompatibility antigen, B alpha chain antibody
    • RT1 class II antigen, Ba chain antibody
    • RT1 class II, locus Ba antibody

Anti-RT1-Ba antibody [MRC OX-3] (Phycoerythrin) 图像

  • Rat Strain: Buffalo
    Isotypic Control: PE Mouse IgG1. Cell Source: Spleen.
    Percentage of cells stained above control: 43.9%

Anti-RT1-Ba antibody [MRC OX-3] (Phycoerythrin) (ab123125)参考文献

This product has been referenced in:
  • Barclay AN Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX 2 antigens. Immunology 44:727-36 (1981). Read more (PubMed: 6119291) »
  • Barclay AN & Mayrhofer G Bone marrow origin of Ia-positive cells in the medulla rat thymus. J Exp Med 153:1666-71 (1981). Read more (PubMed: 6942092) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"