This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

概述

  • 产品名称Anti-RSPO4抗体
    参阅全部 RSPO4 一抗
  • 描述
    兔多克隆抗体to RSPO4

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human RSPO4.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Kidney; Human Testis; Human Ovary; Human Skin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 26 kDa).
ELISA Use a concentration of 1 µg/ml.

靶标

  • 功能Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors.
  • 疾病相关Defects in RSPO4 are the cause of anonychia congenita (ANONC) [MIM:206800]. A rare condition characterized by the absence or severe hypoplasia of all fingernails and toenails without significant bone anomalies.
  • 序列相似性Belongs to the R-spondin family.
    Contains 1 FU (furin-like) repeat.
    Contains 1 TSP type-1 domain.
  • 结构域The FU repeat is required for activation and stabilization of beta-catenin.
  • 翻译后修饰Tyr-112 may be phosphorylated; however as this position is probably extracellular, the vivo relevance is not proven.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C20orf182 antibody
    • CRISTIN4 antibody
    • hRspo4 antibody
    • R-spondin family, member 4 antibody
    • R-spondin-4 antibody
    • Roof plate-specific spondin-4 antibody
    • RSPO4 antibody
    • RSPO4_HUMAN antibody
    see all

Anti-RSPO4 antibody 图像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-RSPO4 antibody (ab98016) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human ovary tissue lysate - total protein (ab30222)
    Lane 4 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,140 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • IHC image of ab98016 staining in human formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab98016, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-RSPO4 antibody (ab98016)参考文献

ab98016 has not yet been referenced specifically in any publications.

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