• 产品名称Anti-RSPO3抗体
    参阅全部 RSPO3 一抗
  • 描述
    山羊多克隆抗体to RSPO3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:


    , corresponding to internal sequence amino acids 181-193 of Human RSPO3 (NP_116173.2). RSPO3(Peptide available as ab135941.)

  • 阳性对照
    • Human skeletal muscle lysate


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab109808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human RSPO3 peptide (ab135941).


  • 功能Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway.
  • 组织特异性Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node.
  • 序列相似性Belongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.
  • 结构域The FU repeats are required for activation and stabilization of beta-catenin.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CRISTIN1 antibody
    • hPWTSR antibody
    • hRspo3 antibody
    • Protein with TSP type-1 repeat antibody
    • PWTSR antibody
    • R-spondin 3 homolog antibody
    • R-spondin-3 [Precursor] antibody
    • R-spondin-3 antibody
    • Roof plate specific spondin-3 antibody
    • Roof plate-specific spondin-3 antibody
    • RSPO3 antibody
    • RSPO3_HUMAN antibody
    • Thrombospondin type I domain containing 2 antibody
    • Thrombospondin type-1 domain-containing protein 2 antibody
    • THSD2 antibody
    see all

Anti-RSPO3 antibody 图像

  • Anti-RSPO3 antibody (ab109808) at 0.3 µg/ml + Human skeletal muscle lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 31 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 hour

Anti-RSPO3 antibody (ab109808)参考文献

ab109808 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse embryo section)
Specification mouse embryo section
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
Blocking step NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Apr 15 2013

I would like to inform you that we have been able to place the RSPO3 peptide used to create ab190808 on to our catalog. The product number for this is ab135945. The datasheet for this product may be found at the following address: http://www.abcam.c...

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Thank you for contacting us.

I have contacted the lab to get this peptide added to our catalog. These may take a few days but I will let you know when it has been added.

I hope this information is helpful to you. Please do not...

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