概述

  • 产品名称Anti-RSPO1抗体
  • 描述
    兔多克隆抗体to RSPO1
  • 特异性At least three isoforms of RSPO1 are known to exist; ab106556 will detect the largest and smallest isoforms. ab106556 will not cross-react with other RSPO family members.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human RSPO1 (N terminal).
    Database link: Q2MKA7

  • 阳性对照
    • Rat spleen tissue lysate; Human spleen tissue, Human heart tissue lysate
  • 常规说明

    Antibodies should not be exposed to prolonged high temperatures.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with RSPO1 peptide (ab214172).
IHC-P Use a concentration of 2.5 - 20 µg/ml.

靶标

  • 功能Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.
  • 组织特异性Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.
  • 疾病相关Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.
  • 序列相似性Belongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.
  • 结构域The FU repeats are required for activation and stabilization of beta-catenin.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CRISTIN3 antibody
    • FLJ40906 antibody
    • hRspo1 antibody
    • R spondin homolog (Xenopus laevis) antibody
    • R spondin homolog antibody
    • R spondin1 antibody
    • R-spondin-1 antibody
    • Roof plate specific spondin antibody
    • Roof plate-specific spondin-1 antibody
    • RP11-566C13.1 antibody
    • RSPO antibody
    • Rspo1 antibody
    • RSPO1_HUMAN antibody
    see all

Anti-RSPO1 antibody 图像

  • Lane 1 : Anti-RSPO1 antibody (ab106556) at 1 µg/ml
    Lane 2 : Anti-RSPO1 antibody (ab106556) at 2 µg/ml

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human heart tissue lysate


    Predicted band size : 29 kDa
  • Immunohistochemical analysis of PFA fixed paraffin embedded Human spleen tissue sections labelling RSPO1 with ab106556, at 2.5 µg/mL. 

  • Lane 1 : Anti-RSPO1 antibody (ab106556) at 1 µg/ml
    Lane 2 : Anti-RSPO1 antibody (ab106556) at 2 µg/ml

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 29 kDa
  • Immunohistochemical analysis of Human spleen tissue labelling RSPO1 with ab106556 at 20 ug/mL.

     

  • Immunohistochemical analysis of PFA fixed paraffin embedded Human heart tissue sections labelling RSPO1 with ab106556, at 2.5 µg/mL. 

Anti-RSPO1 antibody (ab106556)参考文献

ab106556 has not yet been referenced specifically in any publications.

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