概述

  • 产品名称Anti-RSPH3抗体
  • 描述
    兔多克隆抗体to RSPH3
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence corresponding to amino acids 268-354 (IIEVDMECQT DAFLDRPPTP LFIPAKTGKD VATQILEGEL FDFDLEVKPV LEVLVGKTIE QSLLEVMEEE ELANLRASQR EYEELRN) of Human RSPH3, isoform 1.

  • 阳性对照
    • Human testis tissue; RT-4 and U-251 MG cell lysates; Human Plasma, Liver and Tonsil lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

  • 序列相似性Belongs to the flagellar radial spoke RSP3 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ111C20.1 antibody
    • Radial spoke 3 homolog (Chlamydomonas) antibody
    • Radial spoke head 3 homolog antibody
    • Radial spoke head protein 3 homolog antibody
    • Radial spoke head-like protein 2 antibody
    • Radial spoke protein 3 antibody
    • Radial spokehead like 2 antibody
    • RSHL2 antibody
    • RSP3 antibody
    • RSPH3 antibody
    • RSPH3_HUMAN antibody
    see all

Anti-RSPH3 antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122535 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122535, at 1/250 dilution, staining RSPH3 in paraffin-embedded Human testis tissue by Immunohistochemistry, showing moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
  • All lanes : Anti-RSPH3 antibody (ab122535) at 1/250 dilution

    Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma lysate
    Lane 5 : Human Liver lysate
    Lane 6 : Human Tonsil lysate

Anti-RSPH3 antibody (ab122535)参考文献

ab122535 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122535.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"