兔多克隆抗体to RSK1 p90 (phospho T359 + S363)
ab58535 detects endogenous levels of RSK1 p90 only when phosphorylated at threonine 359/serine 363.
Synthetic phosphopeptide derived from human RSK1 p90 around the phosphorylation sites of threonine 359/serine 363 (T
Human breast carcinoma and 293 cell extracts treated with PMA (125ng/ml, 30mins).
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab58535 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against
non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain. Contains 2 protein kinase domains.
Autophosphorylated on Ser-380, as part of the activation process.
Information by UniProt
90 kDa ribosomal protein S6 kinase 1 antibody
dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
Immunohistochemistry (Paraffin-embedded sections) - RSK1 p90 (phospho T359 + S363) antibody (ab58535)
ab58535 at 1/50 dilution staining RSK1 p90 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Western blot - RSK1 p90 (phospho T359 + S363) antibody (ab58535)
All lanes : Anti-RSK1 p90 (phospho T359 + S363) antibody (ab58535) at 1/500 dilution Lane 1 : 293 cell extract treated with PMA (125ng/ml, 30mins) Lane 2 : 293 cell extract treated with PMA (125ng/ml, 30mins) with immunizing peptide Predicted band size : 83 kDa Observed band size : 83 kDa
has not yet been referenced specifically in any publications.
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