重组Anti-RSK1 p90 (phospho S380)抗体[E239] (ab32203)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E239] to RSK1 p90 (phospho S380)
- Suitable for: WB, ICC/IF, IP, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RSK1 p90 (phospho S380)抗体[E239]
参阅全部 RSK1 p90 一抗 -
描述
兔单克隆抗体[E239] to RSK1 p90 (phospho S380) -
宿主
Rabbit -
特异性
The antibody only detects RSK1 phosphorylated on Serine 380. -
经测试应用
适用于: WB, ICC/IF, IP, Dot blotmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IP: C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes. ICC/IF: A431 cells treated with 100ng/ml EGF for 10 minutes. WB: HeLa starved overnight then treated with 200nM TPA for 4 hours whole cell lysate. PC-12 (Rat adrenal gland pheochromocytoma ) treated with 200ng/ml NGF for 24 hours whole cell lysate. NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E239 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32203于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
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ICC/IF |
1/50.
For the unpurified format use at 1/250 - 1/500 dilution |
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IP |
1/20.
For the unpurified format use at 1/80 dilution |
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Dot blot |
1/1000.
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说明 |
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WB
1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa). |
ICC/IF
1/50. For the unpurified format use at 1/250 - 1/500 dilution |
IP
1/20. For the unpurified format use at 1/80 dilution |
Dot blot
1/1000. |
靶标
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功能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Autophosphorylated on Ser-380, as part of the activation process. - Information by UniProt
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数据库链接
- Entrez Gene: 6195 Human
- Entrez Gene: 20111 Mouse
- Entrez Gene: 81771 Rat
- Omim: 601684 Human
- SwissProt: Q15418 Human
- SwissProt: P18653 Mouse
- SwissProt: Q63531 Rat
- Unigene: 149957 Human
see all -
别名
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
see all
图片
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All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) at 1/1000 dilution
Lane 1 : Untreated PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma ) treated with 200ng/ml NGF for 24 hours whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) treated with 200ng/ml NGF for 24 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Image produced using the purified version.
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Purified ab32203 at 1:20 dilution (0.8µg) immunoprecipitating RSK1 p90 in C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes.
Lane 1 (input): C6 (Rat glial tumor glial cell) whole cell lysate treated with 100nM Calyculin A for 30 minutes 10µg.
Lane 2 (+): ab32203 + C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32203 in C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 90 kDa -
Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 10 minutes cells labeling RSK1 p90 with Purified ab32203 at 1:50 dilution (2.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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This data was produced using the unpurified format.
Dot blot analysis of RSK1 p90 (phospho S380) phospho peptide (Lane 1) and RSK1 p90 non-phospho peptide (Lane 2) labeling RSK1 p90 (phospho S380) with ab32203 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 200nM TPA for 4 hours whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 200nM TPA for 4 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Image produced using the purified version.
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All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) at 1/1000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes whole cell lysate, and then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Image produced using the purified version.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab32203 被引用在 13 文献中.
- Stathopoulou K et al. Divergent off-target effects of RSK N-terminal and C-terminal kinase inhibitors in cardiac myocytes. Cell Signal 63:109362 (2019). PubMed: 31344438
- Yang C et al. CXCL1 stimulates migration and invasion in ER-negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis. Int J Oncol 55:684-696 (2019). PubMed: 31322183
- Shrestha D et al. Actin Dysfunction Induces Cell Cycle Delay at G2/M with Sustained ERK and RSK Activation in IMR-90 Normal Human Fibroblasts. Mol Cells 41:436-443 (2018). PubMed: 29754473
- Li FF et al. Pachymic acid protects H9c2 cardiomyocytes from lipopolysaccharide-induced inflammation and apoptosis by inhibiting the extracellular signal-regulated kinase 1/2 and p38 pathways. Mol Med Rep 12:2807-13 (2015). PubMed: 25936656
- Smadja-Lamère N et al. Insulin Activates RSK (p90 Ribosomal S6 Kinase) to Trigger a New Negative Feedback Loop That Regulates Insulin Signaling for Glucose Metabolism. J Biol Chem 288:31165-76 (2013). WB ; Human . PubMed: 24036112